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|a 10.1094/PDIS-05-22-1078-SC
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Medberry, Ava N
|e verfasserin
|4 aut
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| 245 |
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|a A Novel, Divergent Member of the Rhabdoviridae Family Infects Strawberry
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|c 2023
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| 336 |
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|a Text
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|a ƒaComputermedien
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|a Date Completed 05.04.2023
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|a Date Revised 05.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Strawberry (Fragaria × ananassa) is the most important berry crop worldwide and viruses pose a constant threat to the industry. In this communication, we describe a novel virus in the family Rhabdoviridae referred to as strawberry virus 3 (StrV-3). The virus does not show significant homology when compared with recognized rhabdoviruses and, therefore, the establishment of a new genus should be considered. A triplex reverse-transcription PCR test was developed and successfully employed in a survey of the National Clonal Germplasm Repository Fragaria collection. A CRISPR-Cas-based protocol was also developed and shown to detect the virus in as little as 1 fg of total RNA, a protocol to be used in the detection of the virus in candidate G1 plants. The strawberry aphid (Chaetosiphon fragaefolii) was evaluated-alas, unsuccessfully-as a potential vector of the virus. This work broadens our understanding of the family Rhabdoviridae and assists in the quest of releasing plant material free of viruses
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| 650 |
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|a Journal Article
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| 650 |
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4 |
|a CRISPR-Cas
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| 650 |
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4 |
|a RT-PCR
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| 650 |
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4 |
|a Rhabdoviridae
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| 650 |
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4 |
|a certification
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| 650 |
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4 |
|a diagnostics
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| 700 |
1 |
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|a Srivastava, Ashish
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Diaz-Lara, Alfredo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rwahnih, Maher Al
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Villamor, Dan E V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tzanetakis, Ioannis E
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 107(2023), 3 vom: 20. März, Seite 620-623
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|x 0191-2917
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|g volume:107
|g year:2023
|g number:3
|g day:20
|g month:03
|g pages:620-623
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|u http://dx.doi.org/10.1094/PDIS-05-22-1078-SC
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