A LAMP-Based Toolbox Developed for Detecting the Major Pathogens Affecting the Production and Quality of the Chinese Medicinal Crop Aconitum carmichaelii

Aconitum carmichaelii Debeaux is a traditional Chinese medicinal herb that has been utilized for approximately 2,000 years. However, as cultivation has increased, there have been more reports of A. carmichaelii infections caused by four major pathogenic fungal species, Fusarium oxysporum, F. solani,...

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Bibliographische Detailangaben
Veröffentlicht in:	Plant disease. - 1997. - 107(2023), 3 vom: 19. März, Seite 658-666
1. Verfasser:	Liu, Jingzhe (VerfasserIn)
Weitere Verfasser:	Liang, Mengyi, Lin, Tao, Zhao, Qing, Wang, Huiqin, Yang, Shunyuan, Guo, Qian, Wang, Xinyi, Guo, Hua, Cui, Langjun, Yan, Yaping, Hieno, Ayaka, Kageyama, Koji, Suga, Haruhisa, Li, Mingzhu
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2023
Zugriff auf das übergeordnete Werk:	Plant disease
Schlagworte:	Journal Article Aconitum carmichaelii F. solani Fusarium oxysporum Mucor circinelloides Sclerotium rolfsii loop-mediated isothermal amplification (LAMP)

Beschreibung
Zusammenfassung:	Aconitum carmichaelii Debeaux is a traditional Chinese medicinal herb that has been utilized for approximately 2,000 years. However, as cultivation has increased, there have been more reports of A. carmichaelii infections caused by four major pathogenic fungal species, Fusarium oxysporum, F. solani, Mucor circinelloides, and Sclerotium rolfsii, resulting in increased disease incidences and limited production and quality. To detect these infections, we developed a LAMP-based toolbox in this study. The cytochrome c oxidase subunit 1 (cox1) gene, translation elongation factor-1α (EF-1α), internal transcribed spacer (ITS) regions of rDNA, and alcohol dehydrogenase 1 (ADH1) gene, respectively, were used to design species-specific LAMP primer sets for F. oxysporum, F. solani, S. rolfsii, and M. circinelloides. The results showed that the LAMP-based toolbox was effective at detecting pathogens in soil and plant materials. We also used this toolbox to investigate pathogen infection in the main planting regions of A. carmichaelii. Before harvesting, F. oxysporum, M. circinelloides, and S. rolfsii were commonly found in the planting fields and in infected A. carmichaelii plants. Therefore, the toolbox we developed will be useful for tracking these infections, as well as for disease control in A. carmichaelii
Beschreibung:	Date Completed 05.04.2023 Date Revised 05.04.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	0191-2917
DOI:	10.1094/PDIS-05-22-1092-RE