Mitotically heritable epigenetic modifications of CmMYB6 control anthocyanin biosynthesis in chrysanthemum

Mitotically heritable epigenetic modifications of CmMYB6 control anthocyanin biosynthesis in chrysanthemum

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 236(2022), 3 vom: 16. Nov., Seite 1075-1088
1. Verfasser: Tang, Mingwei (VerfasserIn)
Weitere Verfasser: Xue, Wanjie, Li, Xueqi, Wang, Lishan, Wang, Min, Wang, Wanpeng, Yin, Xue, Chen, Bowei, Qu, Xueting, Li, Jingyao, Wu, Yi, Gao, Xinyu, Wei, Xiaofeng, Bu, Fanqi, Zhang, Lingyu, Sui, Zhuoran, Ding, Bing, Wang, Yu, Zhang, Qingzhu, Li, Yuhua, Zhang, Yang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Chrysanthemum CmMYB6 DNA methylation McrBC anthocyanin bisulfite sequencing mitotically heritable Anthocyanins Plant Proteins
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520 |a Flower color, which is determined by various chemical pigments, is a vital trait for ornamental plants, in which anthocyanin is a major component. However, the epigenetic regulation of anthocyanin biosynthesis remains poorly understood. During chrysanthemum cultivation, we found a heterochromatic chrysanthemum accession (YP) whose progeny generated by asexual reproduction contained both yellow-flowered (YP-Y) and pink-flowered (YP-P) plants. In this study, we aimed to elucidate the epigenetic mechanisms of different flower colors in the YP plant progeny. Metabolome and transcriptome analyses revealed that the difference in flower color between YP-Y and YP-P was caused by expression variation of the anthocyanin biosynthesis gene CmMYB6. Bisulfite sequencing revealed that methylation at the CmMYB6 promoter, especially in the CHH context, was higher in YP-Y than YP-P. After demethylation of the CmMYB6 promoter using the dCas9-TET1cd system, the flower color returned from yellow to pink. Furthermore, the methylation status of the CmMYB6 promoter was higher in YP-Y over three consecutive generations, indicating that this methylation status was heritable mitotically. Finally, investigation of other chrysanthemum cultivars showed that the methylation of CmMYB6 decreased gradually with the increase in anthocyanin content. These results lay an epigenetic foundation for the improvement of flower color in horticultural plants 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Chrysanthemum 
650 4 |a CmMYB6 
650 4 |a DNA methylation 
650 4 |a McrBC 
650 4 |a anthocyanin 
650 4 |a bisulfite sequencing 
650 4 |a mitotically heritable 
650 7 |a Anthocyanins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Xue, Wanjie  |e verfasserin  |4 aut 
700 1 |a Li, Xueqi  |e verfasserin  |4 aut 
700 1 |a Wang, Lishan  |e verfasserin  |4 aut 
700 1 |a Wang, Min  |e verfasserin  |4 aut 
700 1 |a Wang, Wanpeng  |e verfasserin  |4 aut 
700 1 |a Yin, Xue  |e verfasserin  |4 aut 
700 1 |a Chen, Bowei  |e verfasserin  |4 aut 
700 1 |a Qu, Xueting  |e verfasserin  |4 aut 
700 1 |a Li, Jingyao  |e verfasserin  |4 aut 
700 1 |a Wu, Yi  |e verfasserin  |4 aut 
700 1 |a Gao, Xinyu  |e verfasserin  |4 aut 
700 1 |a Wei, Xiaofeng  |e verfasserin  |4 aut 
700 1 |a Bu, Fanqi  |e verfasserin  |4 aut 
700 1 |a Zhang, Lingyu  |e verfasserin  |4 aut 
700 1 |a Sui, Zhuoran  |e verfasserin  |4 aut 
700 1 |a Ding, Bing  |e verfasserin  |4 aut 
700 1 |a Wang, Yu  |e verfasserin  |4 aut 
700 1 |a Zhang, Qingzhu  |e verfasserin  |4 aut 
700 1 |a Li, Yuhua  |e verfasserin  |4 aut 
700 1 |a Zhang, Yang  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 236(2022), 3 vom: 16. Nov., Seite 1075-1088  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:236  |g year:2022  |g number:3  |g day:16  |g month:11  |g pages:1075-1088 
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