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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2022.111380
|2 doi
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|a pubmed24n1145.xml
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|a (DE-627)NLM343622947
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|a (NLM)35842058
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|a (PII)S0168-9452(22)00204-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Irfan, Mohammad
|e verfasserin
|4 aut
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|a Fruit ripening specific expression of β-D-N-acetylhexosaminidase (β-Hex) gene in tomato is transcriptionally regulated by ethylene response factor SlERF.E4
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.09.2022
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Published by Elsevier B.V.
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|a N-glycans and N-glycan processing enzymes are key players in regulating the ripening of tomato (Solanum lycopersicum) fruits, a model for fleshy fruit ripening. β-D-N-acetylhexosaminidase (β-Hex) is a N-glycan processing enzyme involved in fruit ripening. The suppression of β-Hex results in enhanced fruit shelf life and firmness in both climacteric and non-climacteric fruits. Previously, we have shown that ripening specific expression of β-Hex is regulated by RIPENING INHIBITOR (RIN), ABSCISIC ACID STRESS RIPENING 1 (SlASR1) and ethylene. However, the precise mechanism of ethylene-mediated regulation of β-Hex remains elusive. To gain insights into this, we have performed 5' deletion mapping of tomato β-Hex promoter and a shorter promoter fragment (pD-200, 200 bp upstream to translational start site) is identified, which was found critical for spatio-temporal transcriptional regulation of β-Hex. Further, site specific mutagenesis in RIN and ASR1 binding sites in pD-200 provides key insights into ripening specific promoter activity. Furthermore, induction of GUS activity by ethylene, yeast one hybrid assay and EMSA identify Ethylene Response Factor SlERF.E4 as a positive regulator of β-Hex. Taken together, our study suggest that SlERF.E4 together with RIN and SlASR1 transcriptionally regulates β-Hex and all these three proteins are essential for fruit ripening specific expression of β-Hex in tomato
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|a Journal Article
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|a Ethylene, Fruit Ripening
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|a Promoter
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|a RIN
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|a SlASR1
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|a SlERF.E4
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|a β-Hex
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|a Ethylenes
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
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|a Kumar, Pankaj
|e verfasserin
|4 aut
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| 700 |
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|a Kumar, Vinay
|e verfasserin
|4 aut
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| 700 |
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|a Datta, Asis
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 323(2022) vom: 01. Okt., Seite 111380
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
1 |
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|g volume:323
|g year:2022
|g day:01
|g month:10
|g pages:111380
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|u http://dx.doi.org/10.1016/j.plantsci.2022.111380
|3 Volltext
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|a AR
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|d 323
|j 2022
|b 01
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|h 111380
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