Monitoring PHB production in Synechocystis sp. with hyperspectral images

Microalgae wastewater treatment systems have the potential for producing added-value products. More specifically, cyanobacteria are able to accumulate polyhydroxybutyrates (PHBs), which can be extracted and used for bioplastics production. Nonetheless, PHB production requires proper culture conditio...

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Veröffentlicht in:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 86(2022), 1 vom: 01. Juli, Seite 211-226
1. Verfasser: Rodríguez Lorenzo, Francisco (VerfasserIn)
Weitere Verfasser: Placer Lorenzo, Miguel, Herrero Castilla, Luz, Álvarez Rodríguez, Juan Antonio, Iglesias, Sandra, Gómez, Santiago, Fernández Montenegro, Juan Manuel, Rueda, Estel, Diez-Montero, Rubén, Garcia, Joan, Gonzalez-Flo, Eva
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Water science and technology : a journal of the International Association on Water Pollution Research
Schlagworte:Journal Article Hydroxybutyrates Polyesters
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520 |a Microalgae wastewater treatment systems have the potential for producing added-value products. More specifically, cyanobacteria are able to accumulate polyhydroxybutyrates (PHBs), which can be extracted and used for bioplastics production. Nonetheless, PHB production requires proper culture conditions and continue monitoring, challenging the state-of-the-art technologies. The aim of this study was to investigate the application of hyperspectral technologies to monitor cyanobacteria population growth and PHB production. We have established a ground-breaking measurement method able to discern spectral reflectance changes from light emitted to cyanobacteria in different phases. All in all, enabling to distinguish between cyanobacteria growth phase and PHB accumulation phase. Furthermore, first tests of classification algorithms used for machine learning and image recognition technologies had been applied to automatically recognize the different cyanobacteria species from a complex microbial community containing cyanobacteria and microalgae cultivated in pilot-scale photobioreactors (PBRs). We have defined three main indicators for monitoring PHB production: (i) cyanobacteria specific-strain density, (ii) differentiate between growth and PHB-accumulation and (iii) chlorosis progression. The results presented in this study represent an interesting alternative for traditional measurements in cyanobacteria PHB production and its application in pilot-scale PBRs. Although not directly determining the amount of PHB production, they would give insights on the undergoing processes 
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650 7 |a Hydroxybutyrates  |2 NLM 
650 7 |a Polyesters  |2 NLM 
700 1 |a Placer Lorenzo, Miguel  |e verfasserin  |4 aut 
700 1 |a Herrero Castilla, Luz  |e verfasserin  |4 aut 
700 1 |a Álvarez Rodríguez, Juan Antonio  |e verfasserin  |4 aut 
700 1 |a Iglesias, Sandra  |e verfasserin  |4 aut 
700 1 |a Gómez, Santiago  |e verfasserin  |4 aut 
700 1 |a Fernández Montenegro, Juan Manuel  |e verfasserin  |4 aut 
700 1 |a Rueda, Estel  |e verfasserin  |4 aut 
700 1 |a Diez-Montero, Rubén  |e verfasserin  |4 aut 
700 1 |a Garcia, Joan  |e verfasserin  |4 aut 
700 1 |a Gonzalez-Flo, Eva  |e verfasserin  |4 aut 
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773 1 8 |g volume:86  |g year:2022  |g number:1  |g day:01  |g month:07  |g pages:211-226 
856 4 0 |u http://dx.doi.org/10.2166/wst.2022.194  |3 Volltext 
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