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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202202913
|2 doi
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|a pubmed24n1143.xml
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|a DE-627
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|a eng
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|a Abbas, Manzar
|e verfasserin
|4 aut
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|a Peptide-Based Coacervate-Core Vesicles with Semipermeable Membranes
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 26.08.2022
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|a Date Revised 26.08.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a Coacervates droplets have long been considered as potential protocells to mimic living cells. However, these droplets lack a membrane and are prone to coalescence, limiting their ability to survive, interact, and organize into higher-order assemblies. This work shows that tyrosine-rich peptide conjugates can undergo liquid-liquid phase separation in a well-defined pH window and transform into stable membrane-enclosed protocells by enzymatic oxidation and cross-linking at the liquid-liquid interface. The oxidation of the tyrosine-rich peptides into dityrosine creates a semipermeable, flexible membrane around the coacervates with tunable thickness, which displays strong intrinsic fluorescence, and stabilizes the coacervate protocells against coalescence. The membranes have an effective molecular weight cut-off of 2.5 kDa, as determined from the partitioning of small dyes and labeled peptides, RNA, and polymers into the membrane-enclosed coacervate protocells. Flicker spectroscopy reveals a membrane bending rigidity of only 0.1kB T, which is substantially lower than phospholipid bilayers despite a larger membrane thickness. Finally, it is shown that enzymes can be stably encapsulated inside the protocells and be supplied with substrates from outside, which opens the way for these membrane-bound compartments to be used as molecularly crowded artificial cells capable of communication or as a vehicle for drug delivery
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|a Journal Article
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|a coacervate-core vesicles
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|a enzyme compartmentalization
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|a liquid-liquid phase separation
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|a membranes
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|a protocells
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|a Peptides
|2 NLM
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|a Polymers
|2 NLM
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|a Tyrosine
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|a 42HK56048U
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|a RNA
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|a 63231-63-0
|2 NLM
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| 700 |
1 |
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|a Law, Jack O
|e verfasserin
|4 aut
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1 |
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|a Grellscheid, Sushma N
|e verfasserin
|4 aut
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| 700 |
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|a Huck, Wilhelm T S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Spruijt, Evan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 34(2022), 34 vom: 07. Aug., Seite e2202913
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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|g volume:34
|g year:2022
|g number:34
|g day:07
|g month:08
|g pages:e2202913
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|u http://dx.doi.org/10.1002/adma.202202913
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