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231226s2022 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2022-0046
|2 doi
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|a pubmed24n1143.xml
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|a (DE-627)NLM343167107
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|a (NLM)35796100
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Cheng, Xiyao
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Premature termination codon
|b a tunable protein translation approach
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| 264 |
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1 |
|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 19.08.2022
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|a Date Revised 01.09.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Cellular protein-protein interactions are largely dependent on the activities of signaling proteins. Here, we present a technique to tune gene expression at translation level based on G418-inducible readthrough premature termination codon (PTC-on). To demonstrate how this PTC-on can control the expression level of a cellular signaling protein to regulate signal transduction, we settled a p53 PTC-on system in p53-null H1299 cells. After treating with G418, the cells expressed full-length p53 protein in a dose-dependent manner. We further demonstrated to use this PTC-on approach to dissect p53-dependent and p53-independent apoptosis in response to the DNA double strand breaks in H1299 cells. In principle, the PTC-on can be used as a general approach for exploring the functions of any other signaling proteins
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|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a MMS
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| 650 |
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4 |
|a apoptosis
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| 650 |
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4 |
|a cell cycle arrest
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| 650 |
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4 |
|a gene expression
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| 650 |
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4 |
|a p53
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| 650 |
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4 |
|a premature termination codon
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| 650 |
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4 |
|a signal transduction
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| 650 |
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4 |
|a translation
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| 650 |
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7 |
|a Codon, Nonsense
|2 NLM
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| 650 |
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7 |
|a Tumor Suppressor Protein p53
|2 NLM
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| 700 |
1 |
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|a Zhou, Ting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Zixin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Jingjing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Meng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Yongqi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Su, Zhengding
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 73(2022), 2 vom: 01. Aug., Seite 80-89
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:73
|g year:2022
|g number:2
|g day:01
|g month:08
|g pages:80-89
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4 |
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|u http://dx.doi.org/10.2144/btn-2022-0046
|3 Volltext
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|d 73
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