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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1107/S1600577522005136
|2 doi
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|a pubmed24n1143.xml
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|a (DE-627)NLM343082489
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|a (NLM)35787568
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ehm, Tamara
|e verfasserin
|4 aut
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|a 3D-printed SAXS chamber for controlled in situ dialysis and optical characterization
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 06.07.2022
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|a Date Revised 16.07.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a open access.
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|a 3D printing changes the scope of how samples can be mounted for small-angle X-ray scattering (SAXS). In this paper a 3D-printed X-ray chamber, which allows for in situ exchange of buffer and in situ optical transmission spectroscopy, is presented. The chamber is made of cyclic olefin copolymers (COC), including COC X-ray windows providing ultra-low SAXS background. The design integrates a membrane insert for in situ dialysis of the 100 µl sample volume against a reservoir, which enables measurements of the same sample under multiple conditions using an in-house X-ray setup equipped with a 17.4 keV molybdenum source. The design's capabilities are demonstrated by measuring reversible structural changes in lipid and polymer systems as a function of salt concentration and pH. In the same chambers optical light transmission spectroscopy was carried out measuring the optical turbidity of the mesophases and local pH values using pH-responsive dyes. Microfluidic exchange and optical spectroscopy combined with in situ X-ray scattering enables vast applications for the study of responsive materials
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|a Journal Article
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|a 3D printing
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|a cyclic olefin copolymer
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|a in situ dialysis
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|a in-house measurements
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|a small-angle X-ray scattering
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|a Philipp, Julian
|e verfasserin
|4 aut
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|a Barkey, Martin
|e verfasserin
|4 aut
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|a Ober, Martina
|e verfasserin
|4 aut
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|a Brinkop, Achim Theo
|e verfasserin
|4 aut
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|a Simml, David
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|4 aut
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|a von Westphalen, Miriam
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|4 aut
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|a Nickel, Bert
|e verfasserin
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|a Beck, Roy
|e verfasserin
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|a Rädler, Joachim O
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 29(2022), Pt 4 vom: 01. Juli, Seite 1014-1019
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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|g volume:29
|g year:2022
|g number:Pt 4
|g day:01
|g month:07
|g pages:1014-1019
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|u http://dx.doi.org/10.1107/S1600577522005136
|3 Volltext
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