Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction

A multitargeted loop-mediated isothermal amplification (MT-LAMP) assay targeting mpt64 (Rv1980c) and IS6110 was designed to diagnose genitourinary tuberculosis (GUTB) cases. While assessing gel-based, hydroxynaphthol blue (HNB) and SYBR Green I MT-LAMP assays on GUTB specimens (n = 28) in a pilot st...

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Veröffentlicht in:	BioTechniques. - 1991. - 73(2022), 1 vom: 27. Juni, Seite 47-57
1. Verfasser:	Kamra, Ekta (VerfasserIn)
Weitere Verfasser:	Singh, Netrapal, Khan, Anish, Singh, Jaideep, Chauhan, Meenakshi, Kamal, Hemant, Mehta, Promod K
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't GUTB HNB dye IS6110 M-PCR MT-LAMP Mtb SYBR Green I dye diagnosis mehr... gel-based LAMP mpt64 Benzothiazoles Diamines Quinolines SYBR Green I 163795-75-3


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100	1		\|a Kamra, Ekta \|e verfasserin \|4 aut
245	1	0	\|a Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction
264		1	\|c 2022
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500			\|a Date Completed 14.07.2022
500			\|a Date Revised 31.07.2022
500			\|a published: Print-Electronic
500			\|a Citation Status MEDLINE
520			\|a A multitargeted loop-mediated isothermal amplification (MT-LAMP) assay targeting mpt64 (Rv1980c) and IS6110 was designed to diagnose genitourinary tuberculosis (GUTB) cases. While assessing gel-based, hydroxynaphthol blue (HNB) and SYBR Green I MT-LAMP assays on GUTB specimens (n = 28) in a pilot study, both gel-based/SYBR Green I assays exhibited better sensitivity than HNB LAMP. Since SYBR Green MT-LAMP is easier to perform compared with a gel-based assay, a higher number of GUTB specimens (n = 55) were evaluated by SYBR Green MT-LAMP, wherein 85.5% sensitivity and 94.4% specificity (n = 36) were obtained. Moreover, the sensitivity attained by MT-LAMP was significantly higher (p < 0.05) than with multiplex-PCR (mpt64 + IS6110). After further validating these MT-LAMP data in different epidemiological settings, this assay may be developed as a diagnostic kit
650		4	\|a Journal Article
650		4	\|a Research Support, Non-U.S. Gov't
650		4	\|a GUTB
650		4	\|a HNB dye
650		4	\|a IS6110
650		4	\|a M-PCR
650		4	\|a MT-LAMP
650		4	\|a Mtb
650		4	\|a SYBR Green I dye
650		4	\|a diagnosis
650		4	\|a gel-based LAMP
650		4	\|a mpt64
650		7	\|a Benzothiazoles \|2 NLM
650		7	\|a Diamines \|2 NLM
650		7	\|a Quinolines \|2 NLM
650		7	\|a SYBR Green I \|2 NLM
650		7	\|a 163795-75-3 \|2 NLM
700	1		\|a Singh, Netrapal \|e verfasserin \|4 aut
700	1		\|a Khan, Anish \|e verfasserin \|4 aut
700	1		\|a Singh, Jaideep \|e verfasserin \|4 aut
700	1		\|a Chauhan, Meenakshi \|e verfasserin \|4 aut
700	1		\|a Kamal, Hemant \|e verfasserin \|4 aut
700	1		\|a Mehta, Promod K \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t BioTechniques \|d 1991 \|g 73(2022), 1 vom: 27. Juni, Seite 47-57 \|w (DE-627)NLM012627046 \|x 1940-9818 \|7 nnas
773	1	8	\|g volume:73 \|g year:2022 \|g number:1 \|g day:27 \|g month:06 \|g pages:47-57
856	4	0	\|u http://dx.doi.org/10.2144/btn-2022-0027 \|3 Volltext
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