Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction

A multitargeted loop-mediated isothermal amplification (MT-LAMP) assay targeting mpt64 (Rv1980c) and IS6110 was designed to diagnose genitourinary tuberculosis (GUTB) cases. While assessing gel-based, hydroxynaphthol blue (HNB) and SYBR Green I MT-LAMP assays on GUTB specimens (n = 28) in a pilot st...

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Veröffentlicht in:BioTechniques. - 1993. - 73(2022), 1 vom: 27. Juni, Seite 47-57
1. Verfasser: Kamra, Ekta (VerfasserIn)
Weitere Verfasser: Singh, Netrapal, Khan, Anish, Singh, Jaideep, Chauhan, Meenakshi, Kamal, Hemant, Mehta, Promod K
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't GUTB HNB dye IS6110 M-PCR MT-LAMP Mtb SYBR Green I dye diagnosis mehr... gel-based LAMP mpt64 Benzothiazoles Diamines Quinolines SYBR Green I 163795-75-3