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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/nph.18350
|2 doi
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|a pubmed24n1143.xml
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|a (DE-627)NLM342967827
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|a (NLM)35775998
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Ludi
|e verfasserin
|4 aut
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| 245 |
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|a Depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 04.11.2022
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|a Date Revised 31.12.2022
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|a published: Print-Electronic
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|a CommentIn: Curr Biol. 2022 Sep 26;32(18):R962-R964. - PMID 36167047
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|a Citation Status MEDLINE
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|a © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
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|a Self-incompatibility (SI) involves specific interactions during pollination to reject incompatible ('self') pollen, preventing inbreeding in angiosperms. A key event observed in pollen undergoing the Papaver rhoeas SI response is the formation of punctate F-actin foci. Pollen tube growth is heavily energy-dependent, yet ATP levels in pollen tubes have not been directly measured during SI. Here we used transgenic Arabidopsis lines expressing the Papaver pollen S-determinant to investigate a possible link between ATP levels, cytosolic pH ([pH]cyt ) and alterations to the actin cytoskeleton. We identify for the first time that SI triggers a rapid and significant ATP depletion in pollen tubes. Artificial depletion of ATP triggered cytosolic acidification and formation of actin aggregates. We also identify in vivo, evidence for a threshold [pH]cyt of 5.8 for actin foci formation. Imaging revealed that SI stimulates acidic cytosolic patches adjacent to the plasma membrane. In conclusion, this study provides evidence that ATP depletion plays a pivotal role in SI upstream of programmed cell death and reveals a link between the cellular energy status, cytosolic acidification and alterations to the actin cytoskeleton in regulating Papaver SI in pollen tubes
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Papaver rhoeas
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| 650 |
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4 |
|a ATP
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| 650 |
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4 |
|a Arabidopsis
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| 650 |
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4 |
|a actin cytoskeleton
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| 650 |
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|a cytosolic acidification
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| 650 |
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4 |
|a pH
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| 650 |
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4 |
|a pollen tubes
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| 650 |
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4 |
|a self-incompatibility
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| 650 |
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7 |
|a Actins
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a Adenosine Triphosphate
|2 NLM
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| 650 |
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7 |
|a 8L70Q75FXE
|2 NLM
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| 700 |
1 |
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|a Lin, Zongcheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carli, José
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gladala-Kostarz, Agnieszka
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Davies, Julia M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Franklin-Tong, Vernonica E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bosch, Maurice
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 236(2022), 5 vom: 07. Dez., Seite 1691-1707
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:236
|g year:2022
|g number:5
|g day:07
|g month:12
|g pages:1691-1707
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| 856 |
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|u http://dx.doi.org/10.1111/nph.18350
|3 Volltext
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|a AR
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|d 236
|j 2022
|e 5
|b 07
|c 12
|h 1691-1707
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