Loss of pod strings in common bean is associated with gene duplication, retrotransposon insertion and overexpression of PvIND

Loss of pod strings in common bean is associated with gene duplication, retrotransposon insertion and overexpression of PvIND

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 235(2022), 6 vom: 25. Sept., Seite 2454-2465
1. Verfasser: Parker, Travis A (VerfasserIn)
Weitere Verfasser: Cetz, Jose, de Sousa, Lorenna Lopes, Kuzay, Saarah, Lo, Sassoum, Floriani, Talissa de Oliveira, Njau, Serah, Arunga, Esther, Duitama, Jorge, Jernstedt, Judy, Myers, James R, Llaca, Victor, Herrera-Estrella, Alfredo, Gepts, Paul
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Phaseolus vulgaris Ty1-copia retrotransposon bHLH transcription factor differential expression domestication gain-of-function mutation gene duplication mehr... pod dehiscence Retroelements
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100 1 |a Parker, Travis A  |e verfasserin  |4 aut 
245 1 0 |a Loss of pod strings in common bean is associated with gene duplication, retrotransposon insertion and overexpression of PvIND 
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500 |a Date Completed 19.08.2022 
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520 |a Fruit development has been central in the evolution and domestication of flowering plants. In common bean (Phaseolus vulgaris), the principal global grain legume staple, two main production categories are distinguished by fibre deposition in pods: dry beans, with fibrous, stringy pods; and stringless snap/green beans, with reduced fibre deposition, which frequently revert to the ancestral stringy state. Here, we identify genetic and developmental patterns associated with pod fibre deposition. Transcriptional, anatomical, epigenetic and genetic regulation of pod strings were explored through RNA-seq, RT-qPCR, fluorescence microscopy, bisulfite sequencing and whole-genome sequencing. Overexpression of the INDEHISCENT ('PvIND') orthologue was observed in stringless types compared with isogenic stringy lines, associated with overspecification of weak dehiscence-zone cells throughout the pod vascular sheath. No differences in DNA methylation were correlated with this phenotype. Nonstringy varieties showed a tandemly direct duplicated PvIND and a Ty1-copia retrotransposon inserted between the two repeats. These sequence features are lost during pod reversion and are predictive of pod phenotype in diverse materials, supporting their role in PvIND overexpression and reversible string phenotype. Our results give insight into reversible gain-of-function mutations and possible genetic solutions to the reversion problem, of considerable economic value for green bean production 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Phaseolus vulgaris 
650 4 |a Ty1-copia retrotransposon 
650 4 |a bHLH transcription factor 
650 4 |a differential expression 
650 4 |a domestication 
650 4 |a gain-of-function mutation 
650 4 |a gene duplication 
650 4 |a pod dehiscence 
650 7 |a Retroelements  |2 NLM 
700 1 |a Cetz, Jose  |e verfasserin  |4 aut 
700 1 |a de Sousa, Lorenna Lopes  |e verfasserin  |4 aut 
700 1 |a Kuzay, Saarah  |e verfasserin  |4 aut 
700 1 |a Lo, Sassoum  |e verfasserin  |4 aut 
700 1 |a Floriani, Talissa de Oliveira  |e verfasserin  |4 aut 
700 1 |a Njau, Serah  |e verfasserin  |4 aut 
700 1 |a Arunga, Esther  |e verfasserin  |4 aut 
700 1 |a Duitama, Jorge  |e verfasserin  |4 aut 
700 1 |a Jernstedt, Judy  |e verfasserin  |4 aut 
700 1 |a Myers, James R  |e verfasserin  |4 aut 
700 1 |a Llaca, Victor  |e verfasserin  |4 aut 
700 1 |a Herrera-Estrella, Alfredo  |e verfasserin  |4 aut 
700 1 |a Gepts, Paul  |e verfasserin  |4 aut 
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773 1 8 |g volume:235  |g year:2022  |g number:6  |g day:25  |g month:09  |g pages:2454-2465 
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