Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 236(2022), 1 vom: 07. Okt., Seite 235-248
1. Verfasser:	Luneau, Julien S (VerfasserIn)
Weitere Verfasser:	Baudin, Maël, Quiroz Monnens, Thomas, Carrère, Sébastien, Bouchez, Olivier, Jardinaud, Marie-Françoise, Gris, Carine, François, Jonas, Ray, Jayashree, Torralba, Babil, Arlat, Matthieu, Lewis, Jennifer D, Lauber, Emmanuelle, Deutschbauer, Adam M, Noël, Laurent D, Boulanger, Alice
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Brassica oleracea Xanthomonas RB-TnSeq fitness hydathodes xylem Bacterial Proteins

Beschreibung
Zusammenfassung:	© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages
Beschreibung:	Date Completed 09.09.2022 Date Revised 15.10.2022 published: Print-Electronic RefSeq: SRP363574, SRP363580 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.18313