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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/nph.18296
|2 doi
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|a pubmed24n1139.xml
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|a (NLM)35653609
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Llavero-Pasquina, Marcel
|e verfasserin
|4 aut
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| 245 |
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|a Thiamine metabolism genes in diatoms are not regulated by thiamine despite the presence of predicted riboswitches
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 03.08.2022
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|a Date Revised 15.10.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
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|a Thiamine pyrophosphate (TPP), an essential co-factor for all species, is biosynthesised through a metabolically expensive pathway regulated by TPP riboswitches in bacteria, fungi, plants and green algae. Diatoms are microalgae responsible for c. 20% of global primary production. They have been predicted to contain TPP aptamers in the 3'UTR of some thiamine metabolism-related genes, but little information is known about their function and regulation. We used bioinformatics, antimetabolite growth assays, RT-qPCR, targeted mutagenesis and reporter constructs to test whether the predicted TPP riboswitches respond to thiamine supplementation in diatoms. Gene editing was used to investigate the functions of the genes with associated TPP riboswitches in Phaeodactylum tricornutum. We found that thiamine-related genes with putative TPP aptamers are not responsive to supplementation with thiamine or its precursor 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), and targeted mutation of the TPP aptamer in the THIC gene encoding HMP-P synthase does not deregulate thiamine biosynthesis in P. tricornutum. Through genome editing we established that PtTHIC is essential for thiamine biosynthesis and another gene, PtSSSP, is necessary for thiamine uptake. Our results highlight the importance of experimentally testing bioinformatic aptamer predictions and provide new insights into the thiamine metabolism shaping the structure of marine microbial communities with global biogeochemical importance
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Phaeodactylum tricornutum
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| 650 |
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|a CRISPR/Cas9
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| 650 |
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|a TPP riboswitch
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| 650 |
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|a aptamer prediction
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|a diatoms
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|a thiamine biosynthesis
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|a thiamine uptake
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|a Riboswitch
|2 NLM
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|a Thiamine Pyrophosphate
|2 NLM
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| 650 |
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|a Q57971654Y
|2 NLM
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| 650 |
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|a Thiamine
|2 NLM
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| 650 |
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|a X66NSO3N35
|2 NLM
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| 700 |
1 |
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|a Geisler, Katrin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Holzer, Andre
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mehrshahi, Payam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mendoza-Ochoa, Gonzalo I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Newsad, Shelby A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Davey, Matthew P
|e verfasserin
|4 aut
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| 700 |
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|a Smith, Alison G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 235(2022), 5 vom: 14. Sept., Seite 1853-1867
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:235
|g year:2022
|g number:5
|g day:14
|g month:09
|g pages:1853-1867
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|u http://dx.doi.org/10.1111/nph.18296
|3 Volltext
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