A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana

A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 235(2022), 6 vom: 07. Sept., Seite 2300-2312
1. Verfasser: Kang, Jingke (VerfasserIn)
Weitere Verfasser: Wang, Xuening, Ishida, Takashi, Grienenberger, Etienne, Zheng, Qian, Wang, Jing, Zhang, Yonghong, Chen, Wenqiang, Chen, Mengmeng, Song, Xiu-Fen, Wu, Chengyun, Hu, Zhubing, Jia, Lingyu, Li, Chen, Liu, Chun-Ming, Fletcher, Jennifer C, Sawa, Shinichiro, Wang, Guodong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis thaliana BAM1 CLE peptide CLV1 shoot regeneration Arabidopsis Proteins Peptides mehr... BAM1 protein, Arabidopsis EC 2.7.11.1 Protein Serine-Threonine Kinases
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500 |a Date Completed 19.08.2022 
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500 |a published: Print-Electronic 
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520 |a © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. 
520 |a Known for their regulatory roles in stem cell homeostasis, CLAVATA3/ESR-RELATED (CLE) peptides also function as mediators of external stimuli such as hormones. De novo shoot regeneration, representing the remarkable plant cellular plasticity, involves reconstitution of stem cells under control of stem-cell regulators. Yet whether and how stem cell-regulating CLE peptides are implicated in plant regeneration remains unknown. By CRISPR/Cas9-induced loss-of-function studies, peptide application, precursor overexpression, and expression analyses, the role of CLE1-CLE7 peptides and their receptors in de novo shoot regeneration was studied in Arabidopsis thaliana. CLE1-CLE7 are induced by callus-induction medium and dynamically expressed in pluripotent callus. Exogenously-applied CLE1-CLE7 peptides or precursor overexpression effectively leads to shoot regeneration suppression, whereas their simultaneous mutation results in enhanced regenerative capacity, demonstrating that CLE1-CLE7 peptides redundantly function as negative regulators of de novo shoot regeneration. CLE1-CLE7-mediated shoot regeneration suppression is impaired in loss-of-function mutants of callus-expressed CLAVATA1 (CLV1) and BARELY ANY MERISTEM1 (BAM1) genes, indicating that CLV1/BAM1 are required for CLE1-CLE7-mediated shoot regeneration signaling. CLE1-CLE7 signaling resulted in transcriptional repression of WUSCHEL (WUS), a stem cell-promoting transcription factor known as a principal regulator of plant regeneration. Our results indicate that functionally-redundant CLE1-CLE7 peptides genetically act through CLV1/BAM1 receptors and repress WUS expression to modulate shoot-regeneration capacity, establishing the mechanistic basis for CLE1-CLE7-mediated shoot regeneration and a novel role for CLE peptides in hormone-dependent developmental plasticity 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Arabidopsis thaliana 
650 4 |a BAM1 
650 4 |a CLE peptide 
650 4 |a CLV1 
650 4 |a shoot regeneration 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Peptides  |2 NLM 
650 7 |a BAM1 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.7.11.1  |2 NLM 
650 7 |a Protein Serine-Threonine Kinases  |2 NLM 
650 7 |a EC 2.7.11.1  |2 NLM 
700 1 |a Wang, Xuening  |e verfasserin  |4 aut 
700 1 |a Ishida, Takashi  |e verfasserin  |4 aut 
700 1 |a Grienenberger, Etienne  |e verfasserin  |4 aut 
700 1 |a Zheng, Qian  |e verfasserin  |4 aut 
700 1 |a Wang, Jing  |e verfasserin  |4 aut 
700 1 |a Zhang, Yonghong  |e verfasserin  |4 aut 
700 1 |a Chen, Wenqiang  |e verfasserin  |4 aut 
700 1 |a Chen, Mengmeng  |e verfasserin  |4 aut 
700 1 |a Song, Xiu-Fen  |e verfasserin  |4 aut 
700 1 |a Wu, Chengyun  |e verfasserin  |4 aut 
700 1 |a Hu, Zhubing  |e verfasserin  |4 aut 
700 1 |a Jia, Lingyu  |e verfasserin  |4 aut 
700 1 |a Li, Chen  |e verfasserin  |4 aut 
700 1 |a Liu, Chun-Ming  |e verfasserin  |4 aut 
700 1 |a Fletcher, Jennifer C  |e verfasserin  |4 aut 
700 1 |a Sawa, Shinichiro  |e verfasserin  |4 aut 
700 1 |a Wang, Guodong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 235(2022), 6 vom: 07. Sept., Seite 2300-2312  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:235  |g year:2022  |g number:6  |g day:07  |g month:09  |g pages:2300-2312 
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