The tomato cytochrome P450 CYP712G1 catalyses the double oxidation of orobanchol en route to the rhizosphere signalling strigolactone, solanacol

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 235(2022), 5 vom: 01. Sept., Seite 1884-1899
1. Verfasser:	Wang, Yanting (VerfasserIn)
Weitere Verfasser:	Durairaj, Janani, Suárez Duran, Hernando G, van Velzen, Robin, Flokova, Kristyna, Liao, Che-Yang, Chojnacka, Aleksandra, MacFarlane, Stuart, Schranz, M Eric, Medema, Marnix H, van Dijk, Aalt D J, Dong, Lemeng, Bouwmeester, Harro J
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't CYP712G1 orobanchol oxidation solanacol strigolactone biosynthesis tomato (Solanum lycopersicum) (4-hydroxy-5,8-dimethyl-3-(4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-3a,4-dihydro-3H-indeno(1,2-b)furan-2(8bH)-one GR24 strigolactone mehr... Heterocyclic Compounds, 3-Ring Lactones Plant Growth Regulators Cytochrome P-450 Enzyme System 9035-51-2

Beschreibung
Zusammenfassung:	© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. Strigolactones (SLs) are rhizosphere signalling molecules and phytohormones. The biosynthetic pathway of SLs in tomato has been partially elucidated, but the structural diversity in tomato SLs predicts that additional biosynthetic steps are required. Here, root RNA-seq data and co-expression analysis were used for SL biosynthetic gene discovery. This strategy resulted in a candidate gene list containing several cytochrome P450s. Heterologous expression in Nicotiana benthamiana and yeast showed that one of these, CYP712G1, can catalyse the double oxidation of orobanchol, resulting in the formation of three didehydro-orobanchol (DDH) isomers. Virus-induced gene silencing and heterologous expression in yeast showed that one of these DDH isomers is converted to solanacol, one of the most abundant SLs in tomato root exudate. Protein modelling and substrate docking analysis suggest that hydroxy-orbanchol is the likely intermediate in the conversion from orobanchol to the DDH isomers. Phylogenetic analysis demonstrated the occurrence of CYP712G1 homologues in the Eudicots only, which fits with the reports on DDH isomers in that clade. Protein modelling and orobanchol docking of the putative tobacco CYP712G1 homologue suggest that it can convert orobanchol to similar DDH isomers as tomato
Beschreibung:	Date Completed 03.08.2022 Date Revised 13.12.2023 published: Print-Electronic RefSeq: OM677764, KAF6168288, KAA8520263, MW384608, MZ476867, MZ476868, MZ476869 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.18272