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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-08-21-1756-RE
|2 doi
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|a eng
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| 100 |
1 |
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|a Guo, Yushuang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Triplex Immunostrip Assay for Rapid Diagnosis of Tobacco Mosaic Virus, Tobacco Vein Banding Mosaic Virus, and Potato Virus Y
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|c 2022
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|a Text
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|a Date Completed 19.12.2022
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Mixed virus infection has increasingly become a problem in the production of Solanaceae crops in recent years; therefore, a fast and accurate detection method is needed. In this study, a novel triplex immunostrip assay was developed for the simultaneous detection of tobacco mosaic virus (TMV), tobacco vein banding mosaic virus (TVBMV), and potato virus Y (PVY). The limits of detection of this novel immunostrip reached 200 ppb (ng/ml), 1 ppm (µg/ml), and 2 ppm for TMV, PVY, and TVBMV particles, respectively. Importantly, no cross-reactivity was observed among TMV, TVBMV, and PVY or to a nontarget virus. When the assay was applied to suspected virus-infected tobacco, tomato, and potato samples collected from fields in Southwest China, samples of single or mixed virus infection were successfully identified. In conclusion, the triplex immunostrip assay provides a fast and easy to use on-site detection method for field epidemiological studies of TMV, TVBMV, and PVY, and for managing diseases that are caused by them
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|a Journal Article
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|a immunostrip
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|a mixed infection
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|a multiplex assay
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| 650 |
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|a rapid diagnosis
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|a virus
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1 |
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|a Wu, Wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xiaolian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jie
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|4 aut
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| 700 |
1 |
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|a Jia, Meng-Ao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tian, Yanping
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
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|g 106(2022), 12 vom: 20. Dez., Seite 3033-3039
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|g year:2022
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|g pages:3033-3039
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|u http://dx.doi.org/10.1094/PDIS-08-21-1756-RE
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