|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM341314110 |
| 003 |
DE-627 |
| 005 |
20231226011506.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/acs.langmuir.1c03238
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1137.xml
|
| 035 |
|
|
|a (DE-627)NLM341314110
|
| 035 |
|
|
|a (NLM)35608952
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Elbaradei, Ahmed
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Oxidation of Membrane Lipids Alters the Activity of the Human Serotonin 1A Receptor
|
| 264 |
|
1 |
|c 2022
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 08.06.2022
|
| 500 |
|
|
|a Date Revised 10.08.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Lipid oxidation has significant effects on lipid bilayer properties; these effects can be expected to extend to interactions between the lipid bilayer and integral membrane proteins. Given that G protein-coupled receptor (GPCR) activity is known to depend on the properties of the surrounding lipid bilayer, these proteins represent an intriguing class of molecules in which the impact of lipid oxidation on protein behavior is studied. Here, we study the effects of lipid oxidation on the human serotonin 1A receptor (5-HT1AR). Giant unilamellar vesicles (GUVs) containing integral 5-HT1AR were fabricated by the hydrogel swelling method; these GUVs contained polyunsaturated 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLinPC) and its oxidation product 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PoxnoPC) at various ratios. 5-HT1AR-integrated GUVs were also fabricated from lipid mixtures that had been oxidized by extended exposure to the atmosphere. Both types of vesicles were used to evaluate 5-HT1AR activity using an assay to quantify GDP-GTP exchange by the coupled G protein α subunit. Results indicated that 5-HT1AR activity increases significantly in bilayers containing oxidized lipids. This work is an important step in understanding how hyperbaric oxidation can change plasma membrane properties and lead to physiological dysfunction
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a HTR1A protein, human
|2 NLM
|
| 650 |
|
7 |
|a Lipid Bilayers
|2 NLM
|
| 650 |
|
7 |
|a Membrane Lipids
|2 NLM
|
| 650 |
|
7 |
|a Phosphatidylcholines
|2 NLM
|
| 650 |
|
7 |
|a Unilamellar Liposomes
|2 NLM
|
| 650 |
|
7 |
|a Receptor, Serotonin, 5-HT1A
|2 NLM
|
| 650 |
|
7 |
|a 112692-38-3
|2 NLM
|
| 650 |
|
7 |
|a Serotonin
|2 NLM
|
| 650 |
|
7 |
|a 333DO1RDJY
|2 NLM
|
| 700 |
1 |
|
|a Wang, Zikai
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Malmstadt, Noah
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 38(2022), 22 vom: 07. Juni, Seite 6798-6807
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:38
|g year:2022
|g number:22
|g day:07
|g month:06
|g pages:6798-6807
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/acs.langmuir.1c03238
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 38
|j 2022
|e 22
|b 07
|c 06
|h 6798-6807
|