|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM341260398 |
| 003 |
DE-627 |
| 005 |
20231226011353.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/cobi.13939
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1137.xml
|
| 035 |
|
|
|a (DE-627)NLM341260398
|
| 035 |
|
|
|a (NLM)35603473
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Kyle, Kathleen E
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Combining surface and soil environmental DNA with artificial cover objects to improve terrestrial reptile survey detection
|
| 264 |
|
1 |
|c 2022
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 01.12.2022
|
| 500 |
|
|
|a Date Revised 13.04.2023
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2022 The Authors. Conservation Biology published by Wiley Periodicals LLC on behalf of Society for Conservation Biology.
|
| 520 |
|
|
|a Reptiles are increasingly of conservation concern due to their susceptibility to habitat loss, emerging disease, and harvest in the wildlife trade. However, reptile populations are often difficult to monitor given the frequency of crypsis in their life history. This difficulty has left uncertain the conservation status of many species and the efficacy of conservation actions unknown. Environmental DNA (eDNA) surveys consistently elevate the detection rate of species they are designed to monitor, and while their use is promising for terrestrial reptile conservation, successes in developing such surveys have been sparse. We tested the degree to which inclusion of surface and soil eDNA sampling into conventional artificial-cover methods elevates the detection probability of a small, cryptic terrestrial lizard, Scincella lateralis. The eDNA sampling of cover object surfaces with paint rollers elevated per sample detection probabilities for this species 4-16 times compared with visual surveys alone. We readily detected S. lateralis eDNA under cover objects up to 2 weeks after the last visual detection, and at some cover objects where no S. lateralis were visually observed in prior months. With sufficient sampling intensity, eDNA testing of soil under cover objects produced comparable per sample detection probabilities as roller surface methods. Our results suggest that combining eDNA and cover object methods can considerably increase the detection power of reptile monitoring programs, allowing more accurate estimates of population size, detection of temporal and spatial changes in habitat use, and tracking success of restoration efforts. Further research into the deposition and decay rates of reptile eDNA under cover objects, as well as tailored protocols for different species and habitats, is needed to bring the technique into widespread use
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a DNAa superficial
|
| 650 |
|
4 |
|a Scincella lateralis
|
| 650 |
|
4 |
|a detection probability
|
| 650 |
|
4 |
|a environmental monitoring
|
| 650 |
|
4 |
|a little brown skink
|
| 650 |
|
4 |
|a monitoreo ambiental
|
| 650 |
|
4 |
|a probabilidad de detección
|
| 650 |
|
4 |
|a surface eDNA
|
| 650 |
|
7 |
|a DNA, Environmental
|2 NLM
|
| 650 |
|
7 |
|a Soil
|2 NLM
|
| 700 |
1 |
|
|a Allen, Michael C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dragon, Jeffrey
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bunnell, John F
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Reinert, Howard K
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zappalorti, Robert
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jaffe, Benjamin D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Angle, Jordan C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lockwood, Julie L
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Conservation biology : the journal of the Society for Conservation Biology
|d 1999
|g 36(2022), 6 vom: 01. Dez., Seite e13939
|w (DE-627)NLM098176803
|x 1523-1739
|7 nnns
|
| 773 |
1 |
8 |
|g volume:36
|g year:2022
|g number:6
|g day:01
|g month:12
|g pages:e13939
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/cobi.13939
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 36
|j 2022
|e 6
|b 01
|c 12
|h e13939
|