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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.2c00464
|2 doi
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|a pubmed24n1136.xml
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|a (DE-627)NLM341015717
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|a (NLM)35578744
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Zhen
|e verfasserin
|4 aut
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| 245 |
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|a Ca2+-Chelation-Induced Fabrication of Multistimuli-Responsive Charged Nanogels from Phospholipid-Polymer Conjugates and Use for Drug/Protein Loading
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 02.06.2022
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|a Date Revised 03.08.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Thermoresponsive phospholipid-poly(N-isopropylacrylamide) (PL-PNIPAM) conjugates were synthesized via reversible addition fragmentation chain transfer polymerization mediated by a phospholipid-modified trithiocarbonate. Temperature triggered the micellization of the PL-PNIPAM conjugate to form phosphate group-decorated micelles in the aqueous solution. Driven by the chelation of phospholipids and Ca2+, the PL-PNIPAM conjugate and Ca2+ ions formed size-tunable nanoclusters at a temperature beyond the lower critical solution temperature. To fabricate cross-linked nanogels, NIPAM was copolymerized with N-succinimidyl acrylate (NSA) to obtain the PL-P(NIPAM-co-NSA) conjugate bearing pendent cross-linkable functionalities. Subsequently, the size-controllable nanogels containing disulfide linkages were generated at 37 °C by cross-linking the PL-P(NIPAM-co-NSA)/Ca2+ nanoclusters with cystamine through modulation of Ca2+ concentrations. These negatively charged nanogels demonstrate temperature/pH/reduction triple responsiveness. The nanogels can be efficiently loaded with doxorubicin (DOX) and proteins with various isoelectric points. The DOX-loaded nanogels exhibited a temperature/pH/reduction triple-responsive release profile. The immobilized RNase A, BSA, and GOx retained the protein bioactivity. The release of RNase A-loaded nanogels possesses a temperature-responsive profile. The immobilization of Lys and cytochrome C in nanogels inhibited protein bioactivity. However, the addition of NaCl triggered the recovery of bioactivity. These multistimuli-responsive nanogels can provide a versatile platform applicable in biotechnology and drug/protein delivery
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Drug Carriers
|2 NLM
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|a Nanogels
|2 NLM
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|a Phospholipids
|2 NLM
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|a Polymers
|2 NLM
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|a Doxorubicin
|2 NLM
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|a 80168379AG
|2 NLM
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|a Ribonuclease, Pancreatic
|2 NLM
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| 650 |
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|a EC 3.1.27.5
|2 NLM
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| 700 |
1 |
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|a Jiang, Yanfen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Hanying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Li
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 38(2022), 21 vom: 31. Mai, Seite 6612-6622
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:38
|g year:2022
|g number:21
|g day:31
|g month:05
|g pages:6612-6622
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|u http://dx.doi.org/10.1021/acs.langmuir.2c00464
|3 Volltext
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