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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erac084
|2 doi
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|a pubmed24n1225.xml
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|a (NLM)35560190
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Hatanaka, Tomoko
|e verfasserin
|4 aut
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|a Different acyl-CoA:diacylglycerol acyltransferases vary widely in function, and a targeted amino acid substitution enhances oil accumulation
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 17.05.2022
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Triacylglycerols (TAGs) are the major component of plant storage lipids such as oils. Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the Kennedy pathway, and is mainly responsible for plant oil accumulation. We previously found that the activity of Vernonia DGAT1 was distinctively higher than that of Arabidopsis and soybean DGAT1 in a yeast microsome assay. In this study, the DGAT1 cDNAs of Arabidopsis, Vernonia, soybean, and castor bean were introduced into Arabidopsis. All Vernonia DGAT1-expressing lines showed a significantly higher oil content (49% mean increase compared with the wild-type) followed by soybean and castor bean. Most Arabidopsis DGAT1-overexpressing lines did not show a significant increase. In addition to these four DGAT1 genes, sunflower, Jatropha, and sesame DGAT1 genes were introduced into a TAG biosynthesis-defective yeast mutant. In the yeast expression culture, DGAT1s from Arabidopsis, castor bean, and soybean only slightly increased the TAG content; however, DGAT1s from Vernonia, sunflower, Jatropha, and sesame increased TAG content >10-fold more than the former three DGAT1s. Three amino acid residues were characteristically common in the latter four DGAT1s. Using soybean DGAT1, these amino acid substitutions were created by site-directed mutagenesis and substantially increased the TAG content
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|a Journal Article
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|a Arabidopsis thaliana
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|a Vernonia galamensis
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|a Acyl-CoA:diacylglycerol acyltransferase
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|a DGAT1
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|a site-directed mutagenesis
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|a triacylglycerol
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|a yeast strain H1246
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|a Acyl Coenzyme A
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Diglycerides
|2 NLM
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|a Plant Oils
|2 NLM
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|a Triglycerides
|2 NLM
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|a DGAT1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a EC 2.3.1.20
|2 NLM
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|a Diacylglycerol O-Acyltransferase
|2 NLM
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| 650 |
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|a EC 2.3.1.20
|2 NLM
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| 700 |
1 |
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|a Tomita, Yoshiki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Matsuoka, Daisuke
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sasayama, Daisuke
|e verfasserin
|4 aut
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| 700 |
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|a Fukayama, Hiroshi
|e verfasserin
|4 aut
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| 700 |
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|a Azuma, Tetsushi
|e verfasserin
|4 aut
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| 700 |
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|a Soltani Gishini, Mohammad Fazel
|e verfasserin
|4 aut
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| 700 |
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|a Hildebrand, David
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 9 vom: 13. Mai, Seite 3030-3043
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:73
|g year:2022
|g number:9
|g day:13
|g month:05
|g pages:3030-3043
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|u http://dx.doi.org/10.1093/jxb/erac084
|3 Volltext
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