OSCA1 is an osmotic specific sensor a method to distinguish Ca2+ -mediated osmotic and ionic perception

OSCA1 is an osmotic specific sensor : a method to distinguish Ca2+ -mediated osmotic and ionic perception

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Détails bibliographiques
Publié dans:The New phytologist. - 1979. - 235(2022), 4 vom: 07. Aug., Seite 1665-1678
Auteur principal: Pei, Songyu (Auteur)
Autres auteurs: Liu, Yuantao, Li, Wenke, Krichilsky, Bryan, Dai, Shiwen, Wang, Yan, Wang, Xi, Johnson, Douglas M, Crawford, Bridget M, Swift, Gary B, Vo-Dinh, Tuan, Pei, Zhen-Ming, Yuan, Fang
Format: Article en ligne
Langue:English
Publié: 2022
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis Ca2+ signaling OSCA1 drought ionic effect osmosensor osmotic effect plus... salt stress Arabidopsis Proteins Calcium Channels OSCA1 protein, Arabidopsis Sodium Chloride 451W47IQ8X Calcium Chloride M4I0D6VV5M Calcium SY7Q814VUP
Description
Résumé:© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
Genetic mutants defective in stimulus-induced Ca2+ increases have been gradually isolated, allowing the identification of cell-surface sensors/receptors, such as the osmosensor OSCA1. However, determining the Ca2+ -signaling specificity to various stimuli in these mutants remains a challenge. For instance, less is known about the exact selectivity between osmotic and ionic stresses in the osca1 mutant. Here, we have developed a method to distinguish the osmotic and ionic effects by analyzing Ca2+ increases, and demonstrated that osca1 is impaired primarily in Ca2+ increases induced by the osmotic but not ionic stress. We recorded Ca2+ increases induced by sorbitol (osmotic effect, OE) and NaCl/CaCl2 (OE + ionic effect, IE) in Arabidopsis wild-type and osca1 seedlings. We assumed the NaCl/CaCl2 total effect (TE) = OE + IE, then developed procedures for Ca2+ imaging, image analysis and mathematic fitting/modeling, and found osca1 defects mainly in OE. The osmotic specificity of osca1 suggests that osmotic and ionic perceptions are independent. The precise estimation of these two stress effects is applicable not only to new Ca2+ -signaling mutants with distinct stimulus specificity but also the complex Ca2+ signaling crosstalk among multiple concurrent stresses that occur naturally, and will enable us to specifically fine tune multiple signal pathways to improve crop yields
Description:Date Completed 15.07.2022
Date Revised 21.07.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18217