Microstructured Hydrogels to Guide Self-Assembly and Function of Lung Alveolospheres

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 34(2022), 28 vom: 13. Juli, Seite e2202992
1. Verfasser:	Loebel, Claudia (VerfasserIn)
Weitere Verfasser:	Weiner, Aaron I, Eiken, Madeline K, Katzen, Jeremy B, Morley, Michael P, Bala, Vikram, Cardenas-Diaz, Fabian L, Davidson, Matthew D, Shiraishi, Kazushige, Basil, Maria C, Ferguson, Laura T, Spence, Jason R, Ochs, Matthias, Beers, Michael F, Morrisey, Edward E, Vaughan, Andrew E, Burdick, Jason A
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article biomaterials hyaluronic acid hydrogels lung organoids Hydrogels Hyaluronic Acid 9004-61-9

Beschreibung
Zusammenfassung:	© 2022 Wiley-VCH GmbH. Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation. Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition). Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres). Using synthetic hydrogels with defined chemical and physical properties, human-induced pluripotent stem cell (iPSC)-derived alveolar type 2 cells (iAT2s) self-assemble into alveolospheres and propagate in Matrigel-free conditions. By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2-derived progenitor cells. This hydrogel system is a facile and accessible system for the culture of iPSC-derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates
Beschreibung:	Date Completed 15.07.2022 Date Revised 03.07.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.202202992