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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erac184
|2 doi
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|a pubmed24n1134.xml
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|a (DE-627)NLM340410396
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|a (NLM)35512445
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Kuang, Weiqi
|e verfasserin
|4 aut
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| 245 |
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|a N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 08.09.2022
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|a Date Revised 20.10.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depending on N supply and time. To quantify the dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was adapted to analyse N mass balance, to image shoot and root growth, and to measure gene expression of Brachypodium distachyon inoculated with the N-fixing bacterium Herbaspirillum seropedicae. Phenotyping throughput of EcoFAB-N was 25-30 plants h-1 with open software and imaging systems. Herbaspirillum seropedicae inoculation of B. distachyon shifted root and shoot growth, nitrate versus ammonium uptake, and gene expression with time; directions and magnitude depended on N availability. Primary roots were longer and root hairs shorter regardless of N, with stronger changes at low N. At higher N, H. seropedicae provided 11% of the total plant N that came from sources other than the seed or the nutrient solution. The time-resolved phenotypic and molecular data point to distinct modes of action: at 5 mM NH4NO3 the benefit appears through N fixation, while at 0.5 mM NH4NO3 the mechanism appears to be plant physiological, with H. seropedicae promoting uptake of N from the root medium.Future work could fine-tune plant and root-associated microorganisms to growth and nutrient dynamics
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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4 |
|a Brachypodium
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|a Herbaspirillum seropedicae
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|a Agriculture
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| 650 |
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|a EcoFAB
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| 650 |
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4 |
|a N fixation
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| 650 |
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|a cereals
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| 650 |
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4 |
|a crop productivity
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| 650 |
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4 |
|a nitrogen
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| 650 |
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4 |
|a non-invasive phenotyping
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| 650 |
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4 |
|a plant-growth promoting rhizobacteria
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| 650 |
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|a wheat
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| 650 |
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|a Ammonium Compounds
|2 NLM
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| 650 |
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|a Nitrates
|2 NLM
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| 700 |
1 |
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|a Sanow, Stefan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kelm, Jana M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Müller Linow, Mark
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Andeer, Peter
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kohlheyer, Dietrich
|e verfasserin
|4 aut
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| 700 |
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|a Northen, Trent
|e verfasserin
|4 aut
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| 700 |
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|a Vogel, John P
|e verfasserin
|4 aut
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| 700 |
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|a Watt, Michelle
|e verfasserin
|4 aut
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| 700 |
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|a Arsova, Borjana
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 15 vom: 03. Sept., Seite 5306-5321
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:73
|g year:2022
|g number:15
|g day:03
|g month:09
|g pages:5306-5321
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|u http://dx.doi.org/10.1093/jxb/erac184
|3 Volltext
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|d 73
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