Profiling of grazed cultures of the chlorophyte alga Dunaliella tertiolecta using an untargeted LC-MS approach

© 2022 Phycological Society of America.

Bibliographische Detailangaben
Veröffentlicht in:Journal of phycology. - 1966. - 58(2022), 4 vom: 18. Aug., Seite 568-581
1. Verfasser: Deore, Pranali (VerfasserIn)
Weitere Verfasser: Barlow, Christopher K, Schittenhelm, Ralf B, Beardall, John, Noronha, Santosh
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Journal of phycology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't algal metabolomics culture crash early warning signals grazer detection
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245 1 0 |a Profiling of grazed cultures of the chlorophyte alga Dunaliella tertiolecta using an untargeted LC-MS approach 
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520 |a Extracellular signals are reported to mediate chemical cross-talk among pelagic microbes, including microalgal prey and predators. Water-soluble mediator compounds play a crucial role in extracellular communication which is vital for prey recognition, attraction, capture, and predator deterrence. A range of exo-metabolites including oxylipins and vitamins are released by prey in response to grazing stress. The temporal dynamics of such exo-metabolites largely remains unknown, especially in large-scale cultivation of microalgae such as closed or open ponds. In open ponds, infestation of predators is almost inevitable but highly undesirable due to the imminent threat of culture collapse. The early production of exo-metabolites emitted by microalgal prey in response to predator attack could be leveraged as diagnostic markers of possible culture collapse. This study uses an untargeted approach for temporal profiling of Dunaliella tertiolecta-specific exo-metabolites under grazing pressure from Oxyrrhis marina. We report 24 putatively identified metabolites, belonging to various classes such as short peptides, lipids, indole-derivatives, and free amino acids, as potential markers of grazing-mediated stress. In addition, this study outlines a clear methodology for screening of exo-metabolites in marine algal samples, the analysis of which is frequently hindered by high salt concentrations. In future, a chemistry-based targeted detection of these metabolites could enable a quick and on-site screening of predators in microalgal cultures 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a algal metabolomics 
650 4 |a culture crash 
650 4 |a early warning signals 
650 4 |a grazer detection 
700 1 |a Barlow, Christopher K  |e verfasserin  |4 aut 
700 1 |a Schittenhelm, Ralf B  |e verfasserin  |4 aut 
700 1 |a Beardall, John  |e verfasserin  |4 aut 
700 1 |a Noronha, Santosh  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of phycology  |d 1966  |g 58(2022), 4 vom: 18. Aug., Seite 568-581  |w (DE-627)NLM098182994  |x 1529-8817  |7 nnns 
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