Transcriptomics uncover the response of an aerobic denitrifying bacteria to zinc oxide nanoparticles exposure
Zinc oxide nanoparticles (ZnO NPs) show adverse impacts on aerobic denitrifying bacteria, little is known about the response of these bacteria to ZnO NPs exposure at cellular level. This study assessed the multiple responses of Pseudomonas aeruginosa PCN-2 under ZnO NPs exposure. We demonstrated tha...
| Publié dans: | Environmental technology. - 1993. - 44(2023), 24 vom: 24. Okt., Seite 3685-3697 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2023
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| Accès à la collection: | Environmental technology |
| Sujets: | Journal Article Aerobic denitrification Pseudomonas aeruginosa ROS ZnO nanoparticles transcriptomics Zinc Oxide SOI2LOH54Z Reactive Oxygen Species |
| Résumé: | Zinc oxide nanoparticles (ZnO NPs) show adverse impacts on aerobic denitrifying bacteria, little is known about the response of these bacteria to ZnO NPs exposure at cellular level. This study assessed the multiple responses of Pseudomonas aeruginosa PCN-2 under ZnO NPs exposure. We demonstrated that ZnO NPs exposure could inhibit the intracellular metabolism and stimulate the antioxidant defence capability of PCN-2. At lower exposure concentration (5 mg/L), exogenous ROS generated and resulted in the inhibition of pyruvate metabolism and citrate cycle, which caused deficient energy for aerobic denitrification. At higher concentrations (50 mg/L), endogenous ROS additionally generated and triggered to stronger down-regulation of oxidative phosphorylation, which caused suppressed electron transfers for aerobic denitrification. Meanwhile, ZnO NPs exposure promoted EPS production and biofilm formation, and antioxidases was especially particularly stimulated at higher concentration. Our findings are significant for understanding of microbial bacterial susceptibility, tolerance and resistance under the exposure of ZnO NPs |
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| Description: | Date Completed 11.09.2023 Date Revised 11.09.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1479-487X |
| DOI: | 10.1080/09593330.2022.2069517 |