Automated Laser-Transfer Synthesis of High-Density Microarrays for Infectious Disease Screening

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 34(2022), 23 vom: 15. Juni, Seite e2200359
1. Verfasser:	Paris, Grigori (VerfasserIn)
Weitere Verfasser:	Heidepriem, Jasmin, Tsouka, Alexandra, Liu, Yuxin, Mattes, Daniela S, Pinzón Martín, Sandra, Dallabernardina, Pietro, Mende, Marco, Lindner, Celina, Wawrzinek, Robert, Rademacher, Christoph, Seeberger, Peter H, Breitling, Frank, Bischoff, Frank Ralf, Wolf, Timo, Loeffler, Felix F
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article Schiff base fluorophores high-throughput laser-induced forward transfer peptides solid phase synthesis Peptides

Beschreibung
Zusammenfassung:	© 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH. Laser-induced forward transfer (LIFT) is a rapid laser-patterning technique for high-throughput combinatorial synthesis directly on glass slides. A lack of automation and precision limits LIFT applications to simple proof-of-concept syntheses of fewer than 100 compounds. Here, an automated synthesis instrument is reported that combines laser transfer and robotics for parallel synthesis in a microarray format with up to 10 000 individual reactions cm- 2 . An optimized pipeline for amide bond formation is the basis for preparing complex peptide microarrays with thousands of different sequences in high yield with high reproducibility. The resulting peptide arrays are of higher quality than commercial peptide arrays. More than 4800 15-residue peptides resembling the entire Ebola virus proteome on a microarray are synthesized to study the antibody response of an Ebola virus infection survivor. Known and unknown epitopes that serve now as a basis for Ebola diagnostic development are identified. The versatility and precision of the synthesizer is demonstrated by in situ synthesis of fluorescent molecules via Schiff base reaction and multi-step patterning of precisely definable amounts of fluorophores. This automated laser transfer synthesis approach opens new avenues for high-throughput chemical synthesis and biological screening
Beschreibung:	Date Completed 10.06.2022 Date Revised 10.06.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.202200359