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20231226003048.0 |
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cr uuu---uuuuu |
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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2022.109008
|2 doi
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|a pubmed24n1131.xml
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|a (DE-627)NLM339510617
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|a (NLM)35421591
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|a (PII)S1521-6616(22)00089-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Du, Hang
|e verfasserin
|4 aut
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1 |
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|a Dok-1 regulates mast cell degranulation negatively through inhibiting calcium-dependent F-actin disassembly
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.05.2022
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|a Date Revised 20.05.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2022. Published by Elsevier Inc.
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|a In food allergies, antigen-induced aggregation of FcεRI on mast cells initiates highly ordered and sequential signaling events. Dok-1(downstream of tyrosine kinase 1), undergoes intense tyrosine phosphorylation upon FcεRI stimulation, which negatively regulates Ras/Erk signaling and the subsequent cytokine release, but it remains unclear whether Dok-1 regulates Fc-mediated degranulation. In this study, we investigated the role of Dok-1 in FcεRI-mediated degranulation. Dok-1 overexpressing RBL-2H3 cells were established. Degranulation, immunoprecipitation, co-immunoprecipitation, immunoblotting and flow cytometry assay were performed to explore the effects of Dok-1 and its underlying mechanisms. We found that, following FcεRI activation, Dok-1 was recruited to the plasma membrane, leading to tyrosine phosphorylation. Phosphorylated Dok-1 inhibits FcεRI-operated calcium influx, and negatively regulated degranulation by inhibiting calcium-dependent disassembly of actin filaments. Our data revealed that Dok-1 is a negative regulator of FcεRI-mediated mast cell degranulation. These findings contribute to the identification of therapeutic targets for food allergies
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Actin filament
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|a Ca(2+)
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|a Downstream of tyrosine kinase
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|a FcεRI
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|a Food allergy
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|a RBL-2H3
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|a Actins
|2 NLM
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|a Receptors, IgE
|2 NLM
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|a Tyrosine
|2 NLM
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|a 42HK56048U
|2 NLM
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Sun, Na
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Han, Shiwen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, Ruolin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Che, Huilian
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 238(2022) vom: 02. Mai, Seite 109008
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:238
|g year:2022
|g day:02
|g month:05
|g pages:109008
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|u http://dx.doi.org/10.1016/j.clim.2022.109008
|3 Volltext
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