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cr uuu---uuuuu |
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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2022.109007
|2 doi
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|a pubmed25n1131.xml
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|a (DE-627)NLM339472588
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|a (NLM)35417749
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|a (PII)S1521-6616(22)00088-2
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lin, Guanbin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Circ_0000854 regulates the progression of hepatocellular carcinoma through miR-1294 /IRGQ axis
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.05.2022
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|a Date Revised 20.05.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2022 Elsevier Inc. All rights reserved.
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|a BACKGROUND: Hepatocellular carcinoma (HCC) is a common cancer disease with the second highest mortality. Circular RNAs (circRNAs) have been shown to play key roles in many tumors, including HCC. However, the function of circ_0000854 in the progression of HCC has not been clarified
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|a METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of circ_0000854, microRNA-1294 (miR-1294) and immunity related GTPase Q (IRGQ) in HCC cells and tissues. Western blot was used for protein expression analysis. Cell processes were detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium Bromide (MTT) assay, thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) assay, transwell assay, flow cytometry, and wound healing assay. Mechanically, the interaction of miR-1294 with circ_0000854 or IRGQ was notarized by dual-luciferase reporter assay and RNA pull-down assay. The xenotransplantation model was established to study the role of circ_0000854 in vivo
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|a RESULTS: Circ_0000854 and IRGQ were highly expressed in HCC tissues and cells, while miR-1294 was downregulated. Silencing circ_0000854 suppressed HCC cell malignant behaviors, including proliferation, cell cycle progression, migration and invasion. Circ_0000854 exhibited sponge effect on miR-1294 and miR-1294 inhibition reversed function of circ_0000854 knockdown. In addition, miR-1294 targeted IRGQ and circ_0000854 sponged miR-1294 to upregulate IRGQ. Overexpression of IRGQ restored miR-1294-induced anti-tumor regulation in HCC cells. Animal experiments confirmed that silencing circ_0000854 inhibited tumor growth and metastasis of HCC via mediating miR-1294 and IRGQ levels in vivo
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|a CONCLUSION: Circ_0000854 accelerated HCC progression via the miR-1294/IRGQ axis, providing a novel regulatory mechanism for HCC pathogenesis
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|a Journal Article
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|a Circ_0000854
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|a Hepatocellular carcinoma
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| 650 |
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|a IRGQ
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|a miR-1294
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| 650 |
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|a MicroRNAs
|2 NLM
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| 700 |
1 |
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|a Li, Jingjing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Kan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Aiping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Chuanyong
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 238(2022) vom: 01. Mai, Seite 109007
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnas
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| 773 |
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|g volume:238
|g year:2022
|g day:01
|g month:05
|g pages:109007
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|u http://dx.doi.org/10.1016/j.clim.2022.109007
|3 Volltext
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