Downregulation of a gibberellin 3β-hydroxylase enhances photosynthesis and increases seed yield in soybean

Downregulation of a gibberellin 3β-hydroxylase enhances photosynthesis and increases seed yield in soybean

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1984. - 235(2022), 2 vom: 20. Juli, Seite 502-517
1. Verfasser: Hu, Dezhou (VerfasserIn)
Weitere Verfasser: Li, Xiao, Yang, Zhongyi, Liu, Shulin, Hao, Derong, Chao, Maoni, Zhang, Jinyu, Yang, Hui, Su, Xiaoyue, Jiang, Mingyue, Lu, Shaoqi, Zhang, Dan, Wang, Li, Kan, Guizhen, Wang, Hui, Cheng, Hao, Wang, Jiao, Huang, Fang, Tian, Zhixi, Yu, Deyue
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't GmGA3ox1 Rubisco activase gibberellin haplotype photosynthesis seed weight seed yield soybean mehr... Gibberellins Mixed Function Oxygenases EC 1.- gibberellin 3beta-hydroxylase EC 1.14.99.-
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500 |a Date Completed 17.06.2022 
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520 |a Seed yield, determined mainly by seed numbers and seed weight, is the primary target of soybean breeding. Identifying the genes underlying yield-related traits is of great significance. Through joint linkage mapping and a genome-wide association study for 100-seed weight, we cloned GmGA3ox1, a gene encoding gibberellin 3β-hydroxylase, which is the key enzyme in the gibberellin synthesis pathway. Genome resequencing identified a beneficial GmGA3ox1 haplotype contributing to high seed weight, which was further confirmed by soybean transformants. CRISPR/Cas9-generated gmga3ox1 mutants showed lower seed weight, but promoted seed yield by increasing seed numbers. The gmga3ox1 mutants reduced gibberellin biosynthesis while enhancing photosynthesis. Knockout of GmGA3ox1 resulted in the upregulation of numerous photosynthesis-related genes, particularly the GmRCA family encoding ribulose-1,5-bispho-sphate carboxylase-oxygenase (Rubisco) activases. The basic leucine zipper transcription factors GmbZIP97 and GmbZIP159, which were both upregulated in the gmga3ox1 mutants and induced by the gibberellin synthesis inhibitor uniconazole, could bind to the promoter of GmRCAβ and activate its expression. Analysis of genomic sequences with over 2700 soybean accessions suggested that GmGA3ox1 is being gradually utilized in modern breeding. Our results elucidated the important role of GmGA3ox1 in soybean yield. These findings reveal important clues for future high-yield breeding in soybean and other crops 
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650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a GmGA3ox1 
650 4 |a Rubisco activase 
650 4 |a gibberellin 
650 4 |a haplotype 
650 4 |a photosynthesis 
650 4 |a seed weight 
650 4 |a seed yield 
650 4 |a soybean 
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700 1 |a Li, Xiao  |e verfasserin  |4 aut 
700 1 |a Yang, Zhongyi  |e verfasserin  |4 aut 
700 1 |a Liu, Shulin  |e verfasserin  |4 aut 
700 1 |a Hao, Derong  |e verfasserin  |4 aut 
700 1 |a Chao, Maoni  |e verfasserin  |4 aut 
700 1 |a Zhang, Jinyu  |e verfasserin  |4 aut 
700 1 |a Yang, Hui  |e verfasserin  |4 aut 
700 1 |a Su, Xiaoyue  |e verfasserin  |4 aut 
700 1 |a Jiang, Mingyue  |e verfasserin  |4 aut 
700 1 |a Lu, Shaoqi  |e verfasserin  |4 aut 
700 1 |a Zhang, Dan  |e verfasserin  |4 aut 
700 1 |a Wang, Li  |e verfasserin  |4 aut 
700 1 |a Kan, Guizhen  |e verfasserin  |4 aut 
700 1 |a Wang, Hui  |e verfasserin  |4 aut 
700 1 |a Cheng, Hao  |e verfasserin  |4 aut 
700 1 |a Wang, Jiao  |e verfasserin  |4 aut 
700 1 |a Huang, Fang  |e verfasserin  |4 aut 
700 1 |a Tian, Zhixi  |e verfasserin  |4 aut 
700 1 |a Yu, Deyue  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1984  |g 235(2022), 2 vom: 20. Juli, Seite 502-517  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnas 
773 1 8 |g volume:235  |g year:2022  |g number:2  |g day:20  |g month:07  |g pages:502-517 
856 4 0 |u http://dx.doi.org/10.1111/nph.18153  |3 Volltext 
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