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231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/jfd.13620
|2 doi
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|a pubmed25n1130.xml
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|a (NLM)35395109
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Chen, Hong
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Exposure of zebrafish to a cold environment triggered cellular autophagy in zebrafish liver
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 10.06.2022
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|a Date Revised 10.06.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 John Wiley & Sons Ltd.
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|a Water temperature is the major ecophysiological factor for fish survival in nature and aquaculture. Compared with many homeotherms, fish can survive prolonged periods under the condition of low temperature. However, the metabolic strategies of the liver under a cold environment are still unknown in this species. In our present study, adult zebrafish were exposed to a cold or cold plus starvation environment to analyse the morphological characteristics of hepatocytes by light microscopy and transmission electron microscopy (TEM). The fish livers were dissected and observed under a microscope, and the liver size and shape appeared normal in all groups. Periodic acid-Schiff and TEM analysis showed that hepatic glycogen was significantly lower in zebrafish exposed to cold acclimation (CF group) than that zebrafish at the control water temperature (CT group). Moreover, qPCR and IHC results indicated that the expression of PYGL (a key enzyme involved in glycogenolysis) markedly increased in the CF group. After cold plus starvation treatment (CS group), autophagy activity was significantly enhanced and numerous mitophagic vacuoles were present in the cytoplasm of hepatocytes. In conclusion, hepatic glycogen was first mobilizing to supply energy, and then autophagy, especially mitophagy, played vital roles during nutrient deprivation in fish species
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|a Journal Article
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| 650 |
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|a Zebrafish
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| 650 |
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|a aquaculture
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| 650 |
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|a autophagy
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| 650 |
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4 |
|a hepatic glycogen
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| 650 |
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4 |
|a temperature
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| 650 |
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7 |
|a Liver Glycogen
|2 NLM
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| 650 |
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7 |
|a Water
|2 NLM
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| 650 |
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7 |
|a 059QF0KO0R
|2 NLM
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| 700 |
1 |
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|a Zhao, Fange
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Kexing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Yihan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liang, Yue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Huiying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Shulin
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 45(2022), 7 vom: 04. Juli, Seite 991-1000
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnas
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| 773 |
1 |
8 |
|g volume:45
|g year:2022
|g number:7
|g day:04
|g month:07
|g pages:991-1000
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/jfd.13620
|3 Volltext
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