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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/nph.18141
|2 doi
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|a pubmed24n1130.xml
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|a (DE-627)NLM339131918
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|a (NLM)35383411
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Choi, Bae Young
|e verfasserin
|4 aut
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| 245 |
1 |
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|a The Chlamydomonas transcription factor MYB1 mediates lipid accumulation under nitrogen depletion
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.06.2022
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|a Date Revised 27.06.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
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|a Microalgae accumulate high levels of oil under stress, but the underlying biosynthetic pathways are not fully understood. We sought to identify key regulators of lipid metabolism under stress conditions. We found that the Chlamydomonas reinhardtii gene encoding the MYB-type transcription factor MYB1 is highly induced under stress conditions. Two myb1 mutants accumulated less total fatty acids and storage lipids than their parental strain upon nitrogen (N) depletion. Transcriptome analysis revealed that genes involved in lipid metabolism are highly enriched in the wild-type but not in the myb1-1 mutant after 4 h of N depletion. Among these genes were several involved in the transport of fatty acids from the chloroplast to the endoplasmic reticulum (ER): acyl-ACP thioesterase (FAT1), Fatty Acid EXporters (FAX1, FAX2), and long-chain acyl-CoA synthetase1 (LACS1). Furthermore, overexpression of FAT1 in the chloroplast increased lipid production. These results suggest that, upon N depletion, MYB1 promotes lipid accumulation by facilitating fatty acid transport from the chloroplast to the ER. This study identifies MYB1 as an important positive regulator of lipid accumulation in C. reinhardtii upon N depletion, adding another player to the established regulators of this process, including NITROGEN RESPONSE REGULATOR 1 (NRR1) and TRIACYLGLYCEROL ACCUMULATION REGULATOR 1 (TAR1)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Chlamydomonas
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4 |
|a MYB transcription factor
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|a acyl-ACP thioesterase
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|a fatty acid biosynthesis
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|a lipid accumulation
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|a nitrogen depletion
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|a triacylglycerol
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|a Fatty Acids
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Triglycerides
|2 NLM
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| 650 |
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Shim, Donghwan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kong, Fantao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Auroy, Pascaline
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Yuree
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li-Beisson, Yonghua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Youngsook
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yamaoka, Yasuyo
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 235(2022), 2 vom: 16. Juli, Seite 595-610
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:235
|g year:2022
|g number:2
|g day:16
|g month:07
|g pages:595-610
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| 856 |
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|u http://dx.doi.org/10.1111/nph.18141
|3 Volltext
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