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cr uuu---uuuuu |
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231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/jfd.13606
|2 doi
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|a pubmed25n1127.xml
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|a (DE-627)NLM338455140
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|a (NLM)35315084
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Huang, Ai-Guo
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Natural component plumbagin as a potential antibacterial agent against Streptococcus agalactiae infection
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| 264 |
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1 |
|c 2022
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 12.05.2022
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|a Date Revised 12.05.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 John Wiley & Sons Ltd.
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|a Streptococcus agalactiae, also known as Group B Streptococcus (GBS), can infect humans, terrestrial animals and fish. The emergence of bacterial resistance of S. agalactiae to antibiotics leads to an urgent need of exploration of new antimicrobial agents. In the study, the antibacterial activity of natural component plumbagin (PLB) against S. agalactiae was investigated in vitro and in vivo. The results showed that the minimal inhibitory concentration (MIC) of PLB against S. agalactiae was 8 mg/L. The growth curve assay revealed that PLB could inhibit the growth of S. agalactiae. In addition, the time-killing curve showed that S. agalactiae was killed almost completely by 2-fold MIC of PLB within 12 h. Transmission electron microscopy results showed obvious severe morphological destruction and abnormal cells of S. agalactiae after treated with PLB. The pathogenicity of S. agalactiae to zebrafish was significantly decreased after preincubation with PLB for 2 h in vitro, further indicating the bactericidal activity of PLB. Interestingly, PLB could kill S. agalactiae without inducing resistance development. Furthermore, pretreatment and post-treatment assays suggested that PLB also exhibited the antibacterial activity against S. agalactiae infection in vivo by effectively reducing the bacterial load and improving the survival rate of S. agalactiae-infected zebrafish. In summary, PLB had potent antibacterial activity against S. agalactiae in vitro and in vivo, and it could be an excellent antimicrobial candidate to prevent and control S. agalactiae infection
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|a Journal Article
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|a Streptococcus agalactiae
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| 650 |
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4 |
|a antimicrobial activity
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| 650 |
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|a efficacy in vivo
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| 650 |
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4 |
|a medicinal plants
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| 650 |
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4 |
|a plumbagin
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| 650 |
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7 |
|a Anti-Bacterial Agents
|2 NLM
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| 650 |
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7 |
|a Naphthoquinones
|2 NLM
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| 650 |
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7 |
|a plumbagin
|2 NLM
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| 650 |
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7 |
|a YAS4TBQ4OQ
|2 NLM
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| 700 |
1 |
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|a Su, Lin-Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a He, Wei-Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Fa-Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wei, Chao-Shuai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Ying-Hui
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 45(2022), 6 vom: 10. Juni, Seite 815-823
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnas
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| 773 |
1 |
8 |
|g volume:45
|g year:2022
|g number:6
|g day:10
|g month:06
|g pages:815-823
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jfd.13606
|3 Volltext
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