Agarose gel electrophoresis to assess PCR product yield : comparison with spectrophotometry, fluorometry and qPCR
Agarose gel electrophoresis is a relatively easy to use method, commonly applied to evaluate PCR reaction success. Intercalating agents or dyes are used to visualize the amplified fragments. However, it is uncertain to what extent the brightness of bands is informative about the concentration of the...
| Veröffentlicht in: | BioTechniques. - 1991. - 72(2022), 4 vom: 05. Apr., Seite 155-158 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2022
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't PCR product quantification agarose gel electrophoresis fluorometry quantitative real-time PCR spectrophotometry Intercalating Agents |
| Online verfügbar |
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