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|a 10.1094/PDIS-01-22-0192-SC
|2 doi
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|a eng
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| 100 |
1 |
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|a Frampton, Rebekah A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Genomes of Potato Mop-Top Virus (Virgaviridae
|b Pomovirus) Isolates from New Zealand and Their Impact on Diagnostic Methods
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 29.09.2022
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|a Date Revised 29.09.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Following the detection of potato mop-top virus (PMTV) in New Zealand in 2018, three near-complete PMTV genomes (AS22, AS99, AS144) were assembled from soil samples taken from potato fields in Canterbury. Phylogenetic analysis revealed that these genomes form a distinct lineage, with limited genetic diversity, within the PMTV species. This analysis supports the hypothesis that these genomes share a common origin, possibly resulting from a single (or limited) incursion of PMTV into New Zealand. A single nucleotide polymorphism was identified in the region where a key diagnostic primer binds. The mismatch of the diagnostic primer has implications for the effectiveness of the Mumford diagnostic protocol currently recommended for use in New Zealand; we recommend that the alternative Pandey assay, for which no primer mismatch was detected, be validated and optimized for use on the viral genomes present in New Zealand
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| 650 |
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|a Journal Article
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|a PMTV
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| 650 |
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|a SNP
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| 650 |
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4 |
|a diagnostics
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| 650 |
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4 |
|a potato
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| 650 |
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|a qPCR
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| 650 |
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4 |
|a virus
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| 650 |
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7 |
|a Soil
|2 NLM
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| 700 |
1 |
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|a Addison, Shea M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kalamorz, Falk
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Smith, Grant R
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 106(2022), 10 vom: 12. Okt., Seite 2571-2575
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|x 0191-2917
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|g volume:106
|g year:2022
|g number:10
|g day:12
|g month:10
|g pages:2571-2575
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|u http://dx.doi.org/10.1094/PDIS-01-22-0192-SC
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