Characterization of trichome-specific BAHD acyltransferases involved in acylsugar biosynthesis in Nicotiana tabacum

Characterization of trichome-specific BAHD acyltransferases involved in acylsugar biosynthesis in Nicotiana tabacum

© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Détails bibliographiques
Publié dans:Journal of experimental botany. - 1985. - 73(2022), 12 vom: 24. Juni, Seite 3913-3928
Auteur principal: Chang, Aixia (Auteur)
Autres auteurs: Hu, Zhongyi, Chen, Biao, Vanderschuren, Herve, Chen, Ming, Qu, Yafang, Yu, Weisong, Li, Yangyang, Sun, Huiqing, Cao, Jianmin, Vasudevan, Kumar, Li, Chenying, Cao, Yanan, Zhang, Jianye, Shen, Yeming, Yang, Aiguo, Wang, Yuanying
Format: Article en ligne
Langue:English
Publié: 2022
Accès à la collection:Journal of experimental botany
Sujets:Journal Article Research Support, Non-U.S. Gov't Nicotiana tabacum (tobacco) NtASAT1 NtASAT2 Acylsugar biosynthesis glandular trichome plant defence Plant Proteins Sucrose plus... 57-50-1 Acyltransferases EC 2.3.-
Description
Résumé:© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
Glandular trichomes of tobacco (Nicotiana tabacum) produce blends of acylsucroses that contribute to defence against pathogens and herbivorous insects, but the mechanism of assembly of these acylsugars has not yet been determined. In this study, we isolated and characterized two trichome-specific acylsugar acyltransferases that are localized in the endoplasmic reticulum, NtASAT1 and NtASAT2. They sequentially catalyse two additive steps of acyl donors to sucrose to produce di-acylsucrose. Knocking out of NtASAT1 or NtASAT2 resulted in deficiency of acylsucrose; however, there was no effect on acylsugar accumulation in plants overexpressing NtASAT1 or NtASAT2. Genomic analysis and profiling revealed that NtASATs originated from the T subgenome, which is derived from the acylsugar-producing diploid ancestor N. tomentosiformis. Our identification of NtASAT1 and NtASAT2 as enzymes involved in acylsugar assembly in tobacco potentially provides a new approach and target genes for improving crop resistance against pathogens and insects
Description:Date Completed 28.06.2022
Date Revised 13.12.2023
published: Print
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/erac095