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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.1c03334
|2 doi
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|a pubmed24n1126.xml
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|a (DE-627)NLM33792158X
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|a (NLM)35261245
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|a DE-627
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|a eng
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|a Sarkar, Deblina
|e verfasserin
|4 aut
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|a Naphthalimide-Based Azo-Functionalized Supramolecular Vesicle in Hypoxia-Responsive Drug Delivery
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 15.04.2022
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|a Date Revised 15.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Supramolecular materials that respond to external triggers are being extensively utilized in developing spatiotemporal control in biomedical applications ranging from drug delivery to diagnostics. The present article describes the development of self-assembled vesicles in 1:9 (v/v), tetrahydrofuran (THF)-water by naphthalimide-based azo moiety containing amphiphile (NI-Azo) where azo moiety would act as the stimuli-responsive junction. The self-assembly of NI-Azo took place through H-type of aggregation. Microscopic and spectroscopic analyses confirmed the formation of supramolecular vesicles with a dimension of 200-250 nm. Azo (-N═N-) moiety is known to get reduced to amine derivatives in the presence of the azoreductase enzyme, which is overexpressed in the hypoxic microenvironment. The absorbance intensity of this characteristic azo (-N═N-) moiety of NI-Azo (1:9 (v/v), THF-water) at 458 nm got diminished in the presence of both extracellular and intracellular bacterial azoreductase extracted from Escherichia coli bacteria. The same observation was noted in the presence of sodium dithionite (mimic of azoreductase), indicating that azoreductase/sodium dithionite induced azo bond cleavage of NI-Azo, which was confirmed by matrix-assisted laser desorption ionization time-of-flight spectrometric data of the corresponding aromatic amine fragments. The anticancer drug, curcumin, was encapsulated inside NI-Azo vesicles that successfully killed B16F10 cells (cancer cells) in CoCl2-induced hypoxic environment owing to the azoreductase-responsive release of drug. The cancer cell killing efficiency by curcumin-loaded NI-Azo vesicles in the hypoxic condition was 2.15-fold higher than that of the normoxic environment and 2.4-fold higher compared to that of native curcumin in the hypoxic condition. Notably, cancer cell killing efficiency of curcumin-loaded NI-Azo vesicles was 4.5- and 1.9-fold higher than that of noncancerous NIH3T3 cells in normoxic and hypoxic environments, respectively. Cell killing was found to be primarily through the early apoptotic pathway
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Amines
|2 NLM
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|a Azo Compounds
|2 NLM
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|a Naphthalimides
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Dithionite
|2 NLM
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| 650 |
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|a 14844-07-6
|2 NLM
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|a Curcumin
|2 NLM
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|a IT942ZTH98
|2 NLM
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|a Chowdhury, Monalisa
|e verfasserin
|4 aut
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|a Das, Prasanta Kumar
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 38(2022), 11 vom: 22. März, Seite 3480-3492
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:38
|g year:2022
|g number:11
|g day:22
|g month:03
|g pages:3480-3492
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|u http://dx.doi.org/10.1021/acs.langmuir.1c03334
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