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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erac093
|2 doi
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|a pubmed25n1126.xml
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|a (DE-627)NLM337901953
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|a (NLM)35259265
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Dong, Chaohua
|e verfasserin
|4 aut
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| 245 |
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|a Apple vacuolar processing enzyme 4 is regulated by cysteine protease inhibitor and modulates fruit disease resistance
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 06.06.2022
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|a Date Revised 03.08.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Ring rot is a destructive apple disease caused by Botryosphaeria dothidea. The resistance mechanism of apple plants to B. dothidea remains unclear. Here, we show that APPLE VACUOLAR PROCESSING ENZYME 4 (MdVPE4) is involved in resistance to B. dothidea. MdVPE4 silencing reduced fruit disease resistance, whereas its overexpression improved resistance. Gene expression analysis revealed that MdVPE4 influenced the expression of fruit disease resistance-related genes, such as APPLE POLYGALACTURONASE 1 (MdPG1), APPLE POLYGALACTURONASE INHIBITOR PROTEIN 1 (MdPGIP1), APPLE ENDOCHITINASE 1 (MdCHI1), and APPLE THAUMATIN-LIKE PROTEIN 1 (MdTHA1). The expression of the four genes responding to B. dothidea infection decreased in MdVPE4-silenced fruits. Further analysis demonstrated that B. dothidea infection induced MdVPE4 expression and enzyme activation in apple fruits. Moreover, MdVPE4 activity was modulated by apple cysteine proteinase inhibitor 1 (MdCPI1), which also contributed to resistance towards B. dothidea, as revealed by gene overexpression and silencing analysis. MdCPI1 interacted with MdVPE4 and inhibited its activity. However, MdCPI1 expression was decreased by B. dothidea infection. Taken together, our findings indicate that the interaction between MdVPE4 and MdCPI1 plays an important role in modulating fruit disease resistance to B. dothidea
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Botryosphaeria dothidea
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|a Malus domestica
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|a cysteine protease inhibitor
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|a fruit disease resistance
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|a ring rot
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|a vacuolar processing enzyme
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|a Cysteine Proteinase Inhibitors
|2 NLM
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|a Polygalacturonase
|2 NLM
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| 650 |
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|a EC 3.2.1.15
|2 NLM
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| 650 |
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|a Cysteine Endopeptidases
|2 NLM
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| 650 |
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|a EC 3.4.22.-
|2 NLM
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| 650 |
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|a vacuolar processing enzyme
|2 NLM
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|a EC 3.4.22.-
|2 NLM
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| 700 |
1 |
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|a Li, Ronghui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Nan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Yingshuang
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Yugang
|e verfasserin
|4 aut
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| 700 |
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|a Bai, Suhua
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 11 vom: 02. Juni, Seite 3758-3773
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
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|g volume:73
|g year:2022
|g number:11
|g day:02
|g month:06
|g pages:3758-3773
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|u http://dx.doi.org/10.1093/jxb/erac093
|3 Volltext
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