Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases
Aims: New methods of DNA recombination that capture the principal advantages of the BioBrick standard (ease of design) and Golden Gate assembly (decreased labor) are demonstrated here. Methods & materials: Both methods employ DNA methyltransferase expression vectors, available from Addgene, that...
| Veröffentlicht in: | BioTechniques. - 1991. - 72(2022), 5 vom: 27. Mai, Seite 185-193 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. BioBrick assembly standard DNA assembly DNA methyltransferase Golden Gate assembly laboratory automation methylase-assisted cloning synthetic biology DNA mehr... |
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