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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1107/S160057752101287X
|2 doi
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|a pubmed24n1511.xml
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|a (NLM)35254315
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|a DE-627
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|e rakwb
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|a eng
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|a Yakovlev, Maksim A
|e verfasserin
|4 aut
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|a A wide-field micro-computed tomography detector
|b micron resolution at half-centimetre scale
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 09.03.2022
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|a Date Revised 24.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a open access.
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|a Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved for fields of view of <2 mm. This article presents a unique detector system with a 6 mm field-of-view image circle and 0.5 µm pixel size that can be used in micro-CT units utilizing both synchrotron and commercial X-ray sources. A resolution-test pattern with linear microstructures and whole adult Daphnia magna were imaged at beamline 8.3.2 of the Berkeley Advanced Light Source. Volumes of 10000 × 10000 × 7096 isotropic 0.5 µm voxels were reconstructed over a 5.0 mm × 3.5 mm field of view. Measurements in the projection domain confirmed a 0.90 µm measured spatial resolution that is largely Nyquist-limited. This unprecedented combination of field of view and resolution dramatically reduces the need for sectional scans and computational stitching for large samples, ultimately offering the means to elucidate changes in tissue and cellular morphology in the context of larger, whole, intact model organisms and specimens. This system is also anticipated to benefit micro-CT imaging in materials science, microelectronics, agricultural science and biomedical engineering
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|a Journal Article
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|a X-ray optics
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|a high resolution
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|a histotomography
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|a large field of view
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|a synchrotron micro-CT
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|a Vanselow, Daniel J
|e verfasserin
|4 aut
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|a Ngu, Mee Siing
|e verfasserin
|4 aut
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|a Zaino, Carolyn R
|e verfasserin
|4 aut
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|a Katz, Spencer R
|e verfasserin
|4 aut
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| 700 |
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|a Ding, Yifu
|e verfasserin
|4 aut
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|a Parkinson, Dula
|e verfasserin
|4 aut
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|a Wang, Steve Yuxin
|e verfasserin
|4 aut
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|a Ang, Khai Chung
|e verfasserin
|4 aut
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|a La Riviere, Patrick
|e verfasserin
|4 aut
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|a Cheng, Keith C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 29(2022), Pt 2 vom: 01. März, Seite 505-514
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
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|g volume:29
|g year:2022
|g number:Pt 2
|g day:01
|g month:03
|g pages:505-514
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|u http://dx.doi.org/10.1107/S160057752101287X
|3 Volltext
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|h 505-514
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