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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.1c03325
|2 doi
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|a pubmed24n1124.xml
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|a (DE-627)NLM337440816
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|a (NLM)35212549
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Ni, Jiaying
|e verfasserin
|4 aut
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| 245 |
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|a Synthesis of Anionic Nanogels for Selective and Efficient Enzyme Encapsulation
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 15.04.2022
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|a Date Revised 15.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Polyelectrolyte nanogels containing cross-linked ionic polymer networks feature both soft environment and intrinsic charges which are of great potential for enzyme encapsulation. In this work, well-defined poly(acrylic acid) (PAA) nanogels have been synthesized based on a facile strategy, namely, electrostatic assembly directed polymerization (EADP). Specifically, AA monomers are polymerized together with a cross-linker in the presence of a cationic-neutral diblock copolymer as the template. Effects of control factors including pH, salt concentration, and cross-linking degree have been investigated systematically, based on which the optimal preparation of PAA nanogels has been established. The obtained nanogel features not only compatible pocket for safely loading enzymes without disturbing their structures, but also abundant negative charges which enable selective and efficient encapsulation of cationic enzymes. The loading capacities of PAA nanogels for cytochrome (cyt c) and lysozyme are 100 and 125 μg/mg (enzyme/nanogel), respectively. More notably, the PAA network seems to modulate a favorable microenvironment for cyt c and induces 2-fold enhanced activity for the encapsulated enzymes, as indicated by the steady-state kinetic assay. Our study reveals the control factors of EADP for optimal synthesis of anionic nanogels and validates their distinctive advances with respect to efficient loading and activation of cationic enzymes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Nanogels
|2 NLM
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|a Polyelectrolytes
|2 NLM
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| 650 |
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|a Polymers
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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| 650 |
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|a 3WJQ0SDW1A
|2 NLM
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| 700 |
1 |
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|a Wan, Yuting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cai, Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Peng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cohen Stuart, Martien A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Junyou
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 38(2022), 10 vom: 15. März, Seite 3234-3243
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:38
|g year:2022
|g number:10
|g day:15
|g month:03
|g pages:3234-3243
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|u http://dx.doi.org/10.1021/acs.langmuir.1c03325
|3 Volltext
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