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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1002/aah.10149
|2 doi
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|a pubmed25n1124.xml
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|a (NLM)35199889
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Zhang, Ting-Ting
|e verfasserin
|4 aut
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| 245 |
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|a Acute Nitrite Exposure Induces Dysfunction and Oxidative Damage in Grass Carp Isolated Hemocytes
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|c 2022
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.06.2022
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|a Date Revised 15.07.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 American Fisheries Society.
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|a To evaluate the effects of nitrite on the oxidative damage of blood cells of Grass Carp Ctenopharyngodon idella, the isolated hemocytes were exposed to nitrite (0, 1, 10, or 100 mg/L) for up to 24 h. Hemoglobin (Hb) and methemoglobin (MetHb) concentrations, reactive oxygen species (ROS) and malondialdehyde (MDA) levels, mitochondrial membrane potential (∆Ψm), and antioxidant enzyme activity were assayed to assess hematological parameters and the antioxidant defense mechanism. Results showed a remarkable decrease in Hb concentration with increasing nitrite concentration after a 24-h exposure, while the MetHb concentration increased significantly in nitrite exposure groups. The levels of ROS, ∆Ψm, and MDA increased to varying degrees with increases in nitrite exposure concentration and time. The total antioxidant capacity, catalase (CAT) activity, glutathione peroxidase (GPx) activity, and glutathione content showed a trend of rising initially and then decreasing with prolonged exposure time. Superoxide dismutase (SOD) activity was higher in the 1-mg/L nitrite exposure group and lower in the 100-mg/L group than in the control. The relative messenger RNA expression ratios of cat, sod1, and gpx were up-regulated significantly in the 1- and 10-mg/L groups and then declined in the 100-mg/L group. Therefore, it can be concluded that nitrite exposure activates the antioxidant defense mechanism of Grass Carp hemocytes and that the balance of oxidant-antioxidant homeostasis will be undermined by higher nitrite doses or longer exposure periods
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Antioxidants
|2 NLM
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| 650 |
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|a Nitrites
|2 NLM
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| 650 |
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|a Reactive Oxygen Species
|2 NLM
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| 700 |
1 |
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|a Ma, Pin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yin, Xiao-Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Dong-Ye
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Da-Peng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Rong
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 34(2022), 2 vom: 04. Juni, Seite 58-68
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnas
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| 773 |
1 |
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|g volume:34
|g year:2022
|g number:2
|g day:04
|g month:06
|g pages:58-68
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|u http://dx.doi.org/10.1002/aah.10149
|3 Volltext
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|d 34
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|h 58-68
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