Biotyping reveals loss of motility in two distinct Yersinia ruckeri lineages exclusive to Norwegian aquaculture

© 2022 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 45(2022), 5 vom: 18. Mai, Seite 641-653
1. Verfasser: Riborg, Andreas (VerfasserIn)
Weitere Verfasser: Colquhoun, Duncan J, Gulla, Snorre
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article Yersinia ruckeri Atlantic salmon aquaculture biotype flagella yersiniosis
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520 |a Non-motile strains of Yersinia ruckeri, known as Y. ruckeri biotype 2, now dominate amongst clinical isolates retrieved from rainbow trout internationally. Due to an acute increase in the number of yersiniosis cases in Norway in recent years, followed by introduction of widespread intraperitoneal vaccination against the disease, an investigation on the prevalence of Y. ruckeri biotype 2 in Norwegian aquaculture was conducted. We biotyped 263 Y. ruckeri isolates recovered from diseased salmonids in Norway between 1985 and 2020. A total of seven biotype 2 isolates were identified, four of which were collected between 1985 and 1987, and three of which belong to the current epizootic clone, isolated from two different sea-farms in 2017. Whole-genome sequencing revealed single non-synonymous nucleotide polymorphisms in the flagellar genes flhC in isolates from the 1980s, and in fliP in isolates from 2017. In both variants, motility was restored both by complementation with wild-type alleles in trans and via spontaneous mutation-driven reversion following prolonged incubation on motility agar. While biotype 2 strains do not yet seem to have become broadly established in Norwegian aquaculture, the seven isolates described here serve to document a further two independent cases of Y. ruckeri biotype 2 emergence in salmonid aquaculture 
650 4 |a Journal Article 
650 4 |a Yersinia ruckeri 
650 4 |a Atlantic salmon 
650 4 |a aquaculture 
650 4 |a biotype 
650 4 |a flagella 
650 4 |a yersiniosis 
700 1 |a Colquhoun, Duncan J  |e verfasserin  |4 aut 
700 1 |a Gulla, Snorre  |e verfasserin  |4 aut 
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