Comparison of optimized methodologies for isolating nuclei from esophageal tissue
Single-nuclei RNA sequencing allows single cell-based analysis in frozen tissue, ameliorating cell recovery biases associated with enzymatic dissociation methods. The authors present two optimized methods for isolating and sequencing nuclei from esophageal tissue using a commercial EZ and citric aci...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 72(2022), 3 vom: 02. März, Seite 104-109
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| 1. Verfasser: |
Kimbley, Lucy M
(VerfasserIn) |
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| Weitere Verfasser: |
Parker, Rachel,
Harrington, Jack,
Walker, Robert C,
Grace, Ben,
West, Jonathan J,
Underwood, Tim J,
Rose-Zerilli, Matthew Jj |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
RNA
esophagus
nuclei
sequencing
single cell
tissue sampling
transcriptome |
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