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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erac022
|2 doi
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|a pubmed25n1122.xml
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|a (DE-627)NLM336672489
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|a (NLM)35134892
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a He, Shutao
|e verfasserin
|4 aut
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| 245 |
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|a Starch synthase II plays a crucial role in starch biosynthesis and the formation of multienzyme complexes in cassava storage roots
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.04.2022
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|a Date Revised 23.05.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Starch is a glucose polymer synthesized by green plants for energy storage and is crucial for plant growth and reproduction. The biosynthesis of starch polysaccharides is mediated by members of the large starch synthase (SS) protein superfamily. Here, we showed that in cassava storage roots, soluble starch synthase II (MeSSII) plays an important role in starch biosynthesis and the formation of protein complexes with other starch biosynthetic enzymes by directly interacting with MeSSI, MeSBEII, and MeISAII. MeSSII-RNAi cassava lines showed increased amylose content and reduced biosynthesis of the intermediate chain of amylopectin (B1 type) in their storage roots, leading to altered starch physicochemical properties. Furthermore, gel permeation chromatography analysis of starch biosynthetic enzymes between wild type and MeSSII-RNAi lines confirmed the key role of MeSSII in the organization of heteromeric starch synthetic protein complexes. The lack of MeSSII in cassava also reduced the capacity of MeSSI, MeSBEII, MeISAI, and MeISAII to bind to starch granules. These findings shed light on the key components of the starch biosynthesis machinery in root crops
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cassava
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|a MeSSII
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|a protein–protein interaction
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|a starch biosynthesis
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|a storage root
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| 650 |
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|a Multienzyme Complexes
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Starch
|2 NLM
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| 650 |
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|a 9005-25-8
|2 NLM
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| 650 |
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|a Amylose
|2 NLM
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| 650 |
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|a 9005-82-7
|2 NLM
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| 650 |
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|a Amylopectin
|2 NLM
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| 650 |
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7 |
|a 9037-22-3
|2 NLM
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| 650 |
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|a starch synthase II
|2 NLM
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| 650 |
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|a EC 2.4.1.-
|2 NLM
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| 650 |
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|a Starch Synthase
|2 NLM
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| 650 |
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|a EC 2.4.1.21
|2 NLM
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| 700 |
1 |
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|a Hao, Xiaomeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Shanshan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Wenzhi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Qiuxiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Xinlu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Luonan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Peng
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 8 vom: 18. Apr., Seite 2540-2557
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
1 |
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|g volume:73
|g year:2022
|g number:8
|g day:18
|g month:04
|g pages:2540-2557
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erac022
|3 Volltext
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|d 73
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|h 2540-2557
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