A structural homologue of the plant receptor D14 mediates responses to strigolactones in the fungal phytopathogen Cryphonectria parasitica

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 234(2022), 3 vom: 15. Mai, Seite 1003-1017
1. Verfasser:	Fiorilli, Valentina (VerfasserIn)
Weitere Verfasser:	Forgia, Marco, de Saint Germain, Alexandre, D'Arrigo, Giulia, Cornu, David, Le Bris, Philippe, Al-Babili, Salim, Cardinale, Francesca, Prandi, Cristina, Spyrakis, Francesca, Boyer, François-Didier, Turina, Massimo, Lanfranco, Luisa
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Cryphonectria parasitica DWARF14 (D14) apocarotenoids fungus perception strigolactones α/β-hydrolase GR24 strigolactone mehr... Heterocyclic Compounds, 3-Ring Lactones Plant Growth Regulators Plant Proteins

Beschreibung
Zusammenfassung:	© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. Strigolactones (SLs) are plant hormones and important signalling molecules required to promote arbuscular mycorrhizal (AM) symbiosis. While in plants an α/β-hydrolase, DWARF14 (D14), was shown to act as a receptor that binds and cleaves SLs, the fungal receptor for SLs is unknown. Since AM fungi are currently not genetically tractable, in this study, we used the fungal pathogen Cryphonectria parasitica, for which gene deletion protocols exist, as a model, as we have previously shown that it responds to SLs. By means of computational, biochemical and genetic analyses, we identified a D14 structural homologue, CpD14. Molecular homology modelling and docking support the prediction that CpD14 interacts with and hydrolyses SLs. The recombinant CpD14 protein shows α/β hydrolytic activity in vitro against the SLs synthetic analogue GR24; its enzymatic activity requires an intact Ser/His/Asp catalytic triad. CpD14 expression in the d14-1 loss-of-function Arabidopsis thaliana line did not rescue the plant mutant phenotype. However, gene inactivation by knockout homologous recombination reduced fungal sensitivity to SLs. These results indicate that CpD14 is involved in SLs responses in C. parasitica and strengthen the role of SLs as multifunctional molecules acting in plant-microbe interactions
Beschreibung:	Date Completed 01.04.2022 Date Revised 31.07.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.18013