Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls

Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 234(2022), 3 vom: 11. Mai, Seite 1031-1046
1. Verfasser: Wu, Di (VerfasserIn)
Weitere Verfasser: Guo, Jianping, Zhang, Qian, Shi, Shaojie, Guan, Wei, Zhou, Cong, Chen, Rongzhi, Du, Bo, Zhu, Lili, He, Guangcun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Nilaparvata lugens Oryza sativa cell wall excretion insect resistance Lignin 9005-53-2
Beschreibung
Zusammenfassung:© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
The planthopper resistance gene Bph6 encodes a protein that interacts with OsEXO70E1. EXO70 forms a family of paralogues in rice. We hypothesized that the EXO70-dependent trafficking pathway affects the excretion of resistance-related proteins, thus impacting plant resistance to planthoppers. Here, we further explored the function of EXO70 members in rice resistance against planthoppers. We used the yeast two-hybrid and co-immunoprecipitation assays to identify proteins that play roles in Bph6-mediated planthopper resistance. The functions of the identified proteins were characterized via gene transformation, plant resistance evaluation, insect performance, cell excretion observation and cell wall component analyses. We discovered that another EXO70 member, OsEXO70H3, interacted with BPH6 and functioned in cell excretion and in Bph6-mediated planthopper resistance. We further found that OsEXO70H3 interacted with an S-adenosylmethionine synthetase-like protein (SAMSL) and increased the delivery of SAMSL outside the cells. The functional impairment of OsEXO70H3 and SAMSL reduced the lignin content and the planthopper resistance level of rice plants. Our results suggest that OsEXO70H3 may recruit SAMSL and help its excretion to the apoplast where it may be involved in lignin deposition in cell walls, thus contributing to rice resistance to planthoppers
Beschreibung:Date Completed 01.04.2022
Date Revised 31.07.2022
published: Print-Electronic
RefSeq: FJ905215, KX818198
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18012