Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 73(2022), 8 vom: 18. Apr., Seite 2308-2319
1. Verfasser:	Wang, Ren (VerfasserIn)
Weitere Verfasser:	Himschoot, Ellie, Grenzi, Matteo, Chen, Jian, Safi, Alaeddine, Krebs, Melanie, Schumacher, Karin, Nowack, Moritz K, Moeder, Wolfgang, Yoshioka, Keiko, Van Damme, Daniël, De Smet, Ive, Geelen, Danny, Beeckman, Tom, Friml, Jiří, Costa, Alex, Vanneste, Steffen
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis PIN protein auxin analogs calcium endosomes root signaling trafficking mehr... Arabidopsis Proteins Indoleacetic Acids Plant Growth Regulators Calcium SY7Q814VUP

Beschreibung
Zusammenfassung:	© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins, and auxin analogs. In this context, synthetic auxin analogs, such as 1-naphthalene acetic acid (1-NAA), are often favored over the endogenous auxin, indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven instrumental in revealing the various faces of auxin, they display in some cases bioactivities distinct from IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in brefeldin A-sensitive endosomal aggregations (BFA bodies), and correlation with the ability to elicit Ca2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin analog-induced Ca2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca2+ response, and their differential ability to elicit Ca2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca2+ signaling does not inhibit BFA body formation in Arabidopsis roots
Beschreibung:	Date Completed 20.04.2022 Date Revised 19.10.2022 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erac019