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231225s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2021.111145
|2 doi
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|a pubmed25n1119.xml
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|a (DE-627)NLM336018746
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|a (NLM)35067308
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|a (PII)S0168-9452(21)00341-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Li, Chao
|e verfasserin
|4 aut
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|a OsLHY is involved in regulating flowering through the Hd1- and Ehd1- mediated pathways in rice (Oryza sativa L.)
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 31.01.2022
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|a Date Revised 31.01.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2021 Elsevier B.V. All rights reserved.
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|a Flowering time (or heading date in crops) is a critical agronomic trait for rice reproduction and adaptation. The circadian clock is an endogenous oscillator that is involved in controlling photoperiodic flowering. The rice LATE ELONGATED HYPOCOTYL (OsLHY), the core oscillator component of circadian clock, is a homolog of the LHY/CCA1 in Arabidopsis. Here we showed that CRISPR/Cas9-engineered mutations in OsLHY caused late flowering in rice only under natural long-day (nLD) and short-day (nSD) conditions, but not artificial SD (10 h light/14 h dark) conditions. In the oslhy mutant, the diurnal expression of circadian clock-related genes was seriously affected under both LD and SD conditions. Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the oslhy mutant under LD conditions. While the transcripts of flowering-related genes were not dramatically influenced under SD conditions. Dual-luciferase assays showed that OsLHY repressed the transcription of OsGI, Hd1, Ghd7, Hd3a, RFT1 and OsELF3, and activated the transcription of Ehd1. Moreover, the yeast one hybrid assay and electrophoretic mobility shift assay confirmed that OsLHY directly repressed OsGI, RFT1 and OsELF3 by binding to their promoters, which is consistent with that in Arabidopsis. These results suggested that the OsLHY can promote rice flowering mainly through regulating Hd1 and Ehd1
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|a Journal Article
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|a Circadian clock
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|a Flowering
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|a OsLHY
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|a Photoperiod pathway
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|a Rice (Oryza sativa)
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Liu, Xue-Jiao
|e verfasserin
|4 aut
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|a Yan, Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Alam, Mohammad Shah
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Zhen
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Zhen-Kun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tao, Ruo-Fu
|e verfasserin
|4 aut
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| 700 |
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|a Yue, Er-Kui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Duan, Ming-Hua
|e verfasserin
|4 aut
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| 700 |
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|a Xu, Jian-Hong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 315(2022) vom: 24. Feb., Seite 111145
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
1 |
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|g volume:315
|g year:2022
|g day:24
|g month:02
|g pages:111145
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|u http://dx.doi.org/10.1016/j.plantsci.2021.111145
|3 Volltext
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|d 315
|j 2022
|b 24
|c 02
|h 111145
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