Calcium-dependent Protein Kinase 5 (CPK5) positively modulates drought tolerance through phosphorylating ABA-Responsive Element Binding Factors in oilseed rape (Brassica napus L.)

Copyright © 2021 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 315(2022) vom: 24. Feb., Seite 111125
1. Verfasser: Cheng, Haokun (VerfasserIn)
Weitere Verfasser: Pan, Gengyu, Zhou, Na, Zhai, Zengkang, Yang, Liuqing, Zhu, Huafan, Cui, Xing, Zhao, Peiyu, Zhang, Hanfeng, Li, Shaojun, Yang, Bo, Jiang, Yuan-Qing
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article ABA-Responsive Element Binding Factors Brassica napus Calcium-dependent protein kinase Drought Phosphorylation G-Box Binding Factors Abscisic Acid 72S9A8J5GW Protein Kinases mehr... EC 2.7.- Calcium SY7Q814VUP
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100 1 |a Cheng, Haokun  |e verfasserin  |4 aut 
245 1 0 |a Calcium-dependent Protein Kinase 5 (CPK5) positively modulates drought tolerance through phosphorylating ABA-Responsive Element Binding Factors in oilseed rape (Brassica napus L.) 
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500 |a Date Completed 31.01.2022 
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520 |a Copyright © 2021 Elsevier B.V. All rights reserved. 
520 |a Drought is an environmental stress that causes severe crop loss. Drought stress can induce abscisic acid (ABA) accumulation and cytoplasmic calcium oscillation. Calcium-dependent protein kinases (CPKs) constitute a group of Ser/Thr protein kinases decoding calcium signals. However, the function and molecular mechanisms of most CPKs in oilseed rape (Brassica napus) remain unknown. Here, we report the functional characterization of BnaCPK5 in drought stress tolerance. BnaCPK5 belongs to Group I of the CPK family and was localized at the plasma membrane and nuclei. Overexpression of BnaCPK5 enhanced drought stress tolerance compared with the control. A screening of interacting proteins identified that BnaCPK5 interacted strongly with two ABA-Responsive Element Binding Factors (ABF/AREBs), BnaABF3 and BnaABF4. BnaCPK5 was shown to phosphorylate both BnaABF3 and BnaABF4 in a kinase assay. Further, it was found that the phosphorylation of BnaABF3 and BnaABF4 by BnaCPK5 increased their transcriptional activities against the famous drought stress marker gene, Responsive to Dehydration (RD) 29B and protein stability. Taken together, these data demonstrate that BnaCPK5 acts as a positive regulator of drought tolerance by, at least in part, phosphorylating two core ABA-signaling components to modulate Late-Embryogenesis Abundant (LEA)-like RD29B expression 
650 4 |a Journal Article 
650 4 |a ABA-Responsive Element Binding Factors 
650 4 |a Brassica napus 
650 4 |a Calcium-dependent protein kinase 
650 4 |a Drought 
650 4 |a Phosphorylation 
650 7 |a G-Box Binding Factors  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a Protein Kinases  |2 NLM 
650 7 |a EC 2.7.-  |2 NLM 
650 7 |a Calcium  |2 NLM 
650 7 |a SY7Q814VUP  |2 NLM 
700 1 |a Pan, Gengyu  |e verfasserin  |4 aut 
700 1 |a Zhou, Na  |e verfasserin  |4 aut 
700 1 |a Zhai, Zengkang  |e verfasserin  |4 aut 
700 1 |a Yang, Liuqing  |e verfasserin  |4 aut 
700 1 |a Zhu, Huafan  |e verfasserin  |4 aut 
700 1 |a Cui, Xing  |e verfasserin  |4 aut 
700 1 |a Zhao, Peiyu  |e verfasserin  |4 aut 
700 1 |a Zhang, Hanfeng  |e verfasserin  |4 aut 
700 1 |a Li, Shaojun  |e verfasserin  |4 aut 
700 1 |a Yang, Bo  |e verfasserin  |4 aut 
700 1 |a Jiang, Yuan-Qing  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant science : an international journal of experimental plant biology  |d 1985  |g 315(2022) vom: 24. Feb., Seite 111125  |w (DE-627)NLM098174193  |x 1873-2259  |7 nnas 
773 1 8 |g volume:315  |g year:2022  |g day:24  |g month:02  |g pages:111125 
856 4 0 |u http://dx.doi.org/10.1016/j.plantsci.2021.111125  |3 Volltext 
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